Population genetics and antimicrobial susceptibility of canine Campylobacter isolates collected before and after a raw feeding experiment

Autor: Anna Hielm-Björkman, Marja-Liisa Hänninen, Rauni Kivistö, Satu Olkkola, Johanna Roine, Sara Kovanen
Přispěvatelé: Departments of Faculty of Veterinary Medicine, Food Hygiene and Environmental Health, Marja-Liisa Hänninen Research Group, Equine and Small Animal Medicine, Petbone – ortopedia, fysioterapia, kivunlievitys, Research Centre for Animal Welfare
Jazyk: angličtina
Rok vydání: 2015
Předmět:
C. COLI
Veterinary medicine
JEJUNI
lcsh:Medicine
medicine.disease_cause
413 Veterinary science
Risk Factors
Zoonoses
Campylobacter Infections
Dog Diseases
lcsh:Science
SEQUENCE TYPING SYSTEM
Finland
education.field_of_study
Multidisciplinary
STREPTOMYCIN RESISTANCE
Campylobacter
3. Good health
Campylobacter upsaliensis
MYCOBACTERIUM-TUBERCULOSIS
Research Article
Genotype
Population
Microbial Sensitivity Tests
Biology
Campylobacter jejuni
Microbiology
Dogs
Antibiotic resistance
Drug Resistance
Bacterial
PET DOGS
PCR ASSAY
medicine
Animals
Humans
Food microbiology
Raw meat
education
RPSL GENE
lcsh:R
biology.organism_classification
Animal Feed
Genetics
Population
Genes
Bacterial
Mutation
Food Microbiology
THERMOPHILIC CAMPYLOBACTER
RISK-FACTORS
Multilocus sequence typing
lcsh:Q
Multilocus Sequence Typing
Zdroj: PLoS ONE, Vol 10, Iss 7, p e0132660 (2015)
PLoS ONE
Popis: In recent years, increasing numbers of consumers have become interested in feeding raw food for their pet dogs as opposed to commercial dry food, in the belief of health advantages. However, raw meat and internal organs, possibly contaminated by pathogens such as Campylobacter spp., may pose a risk of transmission of zoonoses to the pet owners. Campylobacter jejuni is the leading cause of bacterial gastroenteritis in humans but C. upsaliensis has also been associated with human disease. In this study we investigated the effect of different feeding strategies on the prevalence of Campylobacter spp. in Finnish dogs. We further characterized the isolates using multilocus sequence typing (MLST), whole-genome (wg) MLST and antimicrobial susceptibility testing. Dogs were sampled before and after a feeding period consisting of commercial raw feed or dry pellet feed. Altogether 56% (20/36) of the dogs yielded at least one Campylobacter-positive fecal sample. C. upsaliensis was the major species detected from 39% of the dogs before and 30% after the feeding period. Two C. jejuni isolates were recovered, both from raw-fed dogs after the dietary regimen. The isolates represented the same genotype (ST-1326), suggesting a common infection source. However, no statistically significant correlation was found between the feeding strategies and Campylobacter spp. carriage. The global genealogy of MLST types of dog and human C. upsaliensis isolates revealed weakly clonal population structure as most STs were widely dispersed. Major antimicrobial resistance among C. upsaliensis isolates was against streptomycin (STR MIC > 4mg/l). Apart from that, all isolates were highly susceptible against the antimicrobials tested. Mutations were found in the genes rpsL or rpsL and rsmG in streptomycin resistant isolates. In conclusion, increasing trend to feed dogs with raw meat warrants more studies to evaluate the risk associated with raw feeding of pets in transmission of zoonoses to humans.
Databáze: OpenAIRE
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