Effect of Thymoquinone on Oxidative Stress in Escherichia coli–Induced Pyelonephritis in Rats
| Autor: | Omer Evirgen, Ahmet Gokce, Gökçe, Ahmet, Oktay Hasan Ozturk, Emel Nacar, Yusuf Onlen, Burcin Ozer, Vicdan Koksaldi Motor |
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| Přispěvatelé: | Evirgen, O, Gokce, A, Ozturk, OH, Nacar, E, Onlen, Y, Ozer, B, Motor, VK, Sakarya Üniversitesi/Tıp Fakültesi/Cerrahi Tıp Bilimleri Bölümü, Gökçe, Ahmet |
| Rok vydání: | 2011 |
| Předmět: |
kidney
Antioxidant Urinary system medicine.medical_treatment thymoquinone Pharmacology urologic and male genital diseases medicine.disease_cause chemistry.chemical_compound Diabetes mellitus medicine oxidative stress Pharmacology (medical) Pharmacology & Pharmacy Escherichia coli Thymoquinone Original Research Kidney Renal damage business.industry medicine.disease humanities Wistar rats medicine.anatomical_structure chemistry Immunology pyelonephritis business Oxidative stress |
| Zdroj: | Current Therapeutic Research. 72:204-215 |
| ISSN: | 0011-393X |
| Popis: | BackgroundRecurrent urinary tract infections are important in children and adults with diabetes mellitus and/or incontinence due to risk of pyelonephritis (PYN) and renal damage. There is a positive correlation released free radicals during PYN and renal damage. Experimental studies showed that antioxidant agents improve renal damage when used immediately after bacterial inoculation.ObjectiveThe aim of the present study was to evaluate whether treatment by thymoquinone (TQ) before or during Escherichia coli inoculation prevents oxidative damage in acute pyelonephritis (PYN) in an ascending obstructive rat model.MethodsIn this study, 42 Wistar rats were grouped as follows: control, PYN (24, 48, and 72 hours), and TQ-PYN (24, 48, and 72 hours). E. coli (1 ×109 colony forming units) was inoculated into the bladder via urethral catheterization in both the PYN and TQ groups. TQ injections were performed 24 hours before bacteria inoculation and repeated at 24-hour intervals during the indicated time at a dose of 10 mg/kg body weight intraperitoneally in TQ groups.ResultsSuperoxide dismutase activity was statistically lower in the TQ-PYN-48 and -72 groups than the PYN-48 and -72 groups (P < 0.001, P = 0.004, respectively). Catalase activity was significantly higher in PYN-24, -48, and -72 groups than the control group (P < 0.001). In addition, there was a significant difference between the TQ-PYN-24, -48, and -72 groups and PYN groups in terms of glutathione peroxidase activity (P < 0.001, P = 0.026, P = 0.046, respectively). When the TQ-PYN-72 group was compared with the PYN-72 group, malondialdehyde levels were significantly lower in the TQ-PYN-72 group than in the PYN-72 group (P = 0.033). A histologic examination also confirmed the protective effect of TQ. In statistical analysis of histopathologic findings, there were significant differences between the PYN-24 and TQ-PYN-24, PYN-48 and TQ-PYN-48, and PYN-72 and TQ-PYN-72 groups (P = 0.008, P < 0.001, P < 0.001, respectively).ConclusionsThe results indicate that TQ administration attenuated the oxidative damage that occurred in PYN and, therefore, could be used as a supportive agent to protect the kidneys from oxidative damage caused by PYN. |
| Databáze: | OpenAIRE |
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