Serum proteins on nanoparticles: early stages of the 'protein corona'
| Autor: | Kathleen P. Wilkie, Sarah McColman, David T. Cramb, Rui Li, Amanda Bishop, Selena Osman |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
chemistry.chemical_classification
biology Biomolecule Nanoparticle Serum Albumin Bovine Protein Corona Plasma protein binding chemistry.chemical_compound Förster resonance energy transfer chemistry Quantum Dots Monolayer biology.protein Biophysics Nanoparticles Polystyrenes General Materials Science Polystyrene Bovine serum albumin |
| Zdroj: | Nanoscale. 13:20550-20563 |
| ISSN: | 2040-3372 2040-3364 |
| DOI: | 10.1039/d1nr06137b |
| Popis: | Nanoparticles in biological systems such as the bloodstream are exposed to a complex solution of biomolecules. A "corona" monolayer of proteins has historically been thought to form on nanoparticles upon introduction into such environments. To examine the first steps of protein binding, Fluorescence Correlation/Cross Correlation Spectroscopy and Fluorescence Resonance Energy Transfer were used to directly analyze four different nanoparticle systems. CdSe/ZnS core/shell quantum dots, 100 nm diameter polystyrene fluospheres, 200 nm diameter polystyrene fluospheres, and 200 nm diameter PEG-grafted DOTAP liposomes were studied with respect to serum protein binding, using bovine serum albumin as a model. Surface heterogeneity is found to be a key factor in protein binding to these nanoparticles, and as such we present a novel conceptualization of the early hard corona as low-ratio, non-uniform binding rather than a uniform monolayer. |
| Databáze: | OpenAIRE |
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