Presence of a reversible inhibitor(s) for human lymphoid cell RNA, protein, and DNA synthesis in the extracts of established human lymphoid cell lines
| Autor: | Philip R. Glade, Photini S. Papageorgiou, Carol F. Sorokin, Lance M. Tibbetts |
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| Rok vydání: | 1974 |
| Předmět: |
Hot Temperature
Immunology Cell Biology Lymphocyte Activation Tritium Cell Line HeLa Leucine Culture Techniques Lectins medicine Humans Trypsin Carbon Radioisotopes Infectious Mononucleosis Lymphocytes Cytotoxicity Lymphotoxin-alpha Uridine Skin Deoxyribonucleases DNA synthesis Cell-Free System Tissue Extracts Muscles RNA DNA Hydrogen-Ion Concentration biology.organism_classification Molecular biology Embryonic stem cell Lymphotoxin medicine.anatomical_structure Cell culture Protein Biosynthesis Cell Migration Inhibition Thymidine |
| Zdroj: | Cellular immunology. 11(1-3) |
| ISSN: | 0008-8749 |
| Popis: | An endogenous inhibitor for human lymphocyte RNA, protein, and DNA synthesis contained in the extracts of cultured human lymphoid cells (PGLC-33H) is described. The cell extract was found to inhibit RNA, protein and DNA synthesis of autologous cultured lymphoid cells (PGLC-33H) as well as heterologous PHA stimulated human peripheral lymphocytes but not that of nonlymphoid cells (HeLa cells, human embryonic lung, human embryonic skin and muscle and WI-38). Extracts of human peripheral lymphocytes were also found to inhibit the DNA synthesis of cultured lymphoid cells (PGLC-33H) and PHA stimulated human peripheral lymphocytes but not that of nonlymphoid cells (HeLa cells and human embryonic lung). Similarly prepared extracts of long term cultured skin and muscle (H 1 S) cells did not inhibit the DNA synthesis of the cultured lymphoid cells. No evidence of cytotoxicity was detected, and the inhibition was reversible. The inhibitory activity of the extract was found to be thermolabile, nondialyzable, DNase resistant, and sensitive to trypsin. The inhibitor seems to be different from lymphotoxin. |
| Databáze: | OpenAIRE |
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