Effects of Chronic Hypoxia on Opioid Peptide and Catecholamine Levels and on the Release of Dopamine in the Rabbit Carotid Body

Autor: P. R. González-Guerrero, Ricardo Rigual, Constancio Gonzalez
Rok vydání: 1993
Předmět:
Zdroj: Journal of Neurochemistry. 60:1769-1776
ISSN: 1471-4159
0022-3042
DOI: 10.1111/j.1471-4159.1993.tb13402.x
Popis: Carotid body catecholamine and opioid levels were measured in rabbits exposed for 8 days to an atmosphere of 11% O2 in N2 (PO2 of approximately 80 mm Hg) and during an identical period of recovery, i.e., after 8 days of returning to the control normoxic atmosphere. Carotid bodies show a decrease in dopamine content at day 2. Thereafter, the levels of this biogenic amine increase progressively to peak at day 10, that is, 2 days after returning to a normoxic atmosphere. Finally, dopamine levels start to decrease and reach prehypoxic control levels at day 16, that is, after 8 days of recovery. In contrast, levels of native opioid peptides remain unchanged during the whole duration of the experiment, except for a decrease at day 2 of the hypoxic exposure. Levels of total opioid peptides are also below control values at day 2 of hypoxia, increase above control values on returning to a normoxic atmosphere (maximal levels at days 10-12), and later decrease to reach prehypoxic levels at day 16. As a result of these changes the ratios of dopamine to opioid levels show a progressive increase from day 0 to day 10 of the experiment and then return to control prehypoxic values. Carotid bodies isolated from animals that have been exposed to hypoxia for 8 days synthesize [3H]dopamine from its natural precursor [3H]tyrosine at a rate of 175 pmol/mg of protein/h, which is about double the rate of synthesis found in the carotid bodies of control animals and those allowed to recover for 8 days.(ABSTRACT TRUNCATED AT 250 WORDS)
Databáze: OpenAIRE
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