Casticin impairs cell migration and invasion of mouse melanoma B16F10 cellsviaPI3K/AKT and NF-κB signaling pathways
| Autor: | Hsiu Chen Chou, Hung Sheng Shang, Hsiao Min Chou, Yung Luen Shih, Jing Gung Chung, Yung Lin Chu, Hsu Feng Lu |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Health Toxicology and Mutagenesis Melanoma Experimental Antineoplastic Agents Apoptosis Management Monitoring Policy and Law Biology Toxicology Mice Phosphatidylinositol 3-Kinases 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Gentamicin protection assay Cell Movement Cell Line Tumor Animals Humans Neoplasm Invasiveness MTT assay Viability assay Protein kinase B Flavonoids Cell adhesion molecule NF-kappa B Cell migration General Medicine Cell biology Vascular endothelial growth factor A 030104 developmental biology Matrix Metalloproteinase 9 chemistry 030220 oncology & carcinogenesis Casticin Matrix Metalloproteinase 2 Proto-Oncogene Proteins c-akt Signal Transduction |
| Zdroj: | Environmental Toxicology. 32:2097-2112 |
| ISSN: | 1520-4081 |
| Popis: | Casticin, a polymethoxyflavone, is one of the major active components obtained from Fructus viticis, which have been shown to have anticancer activities including induce cell apoptosis in human cancer cells. The aim of this study was to investigate the molecular mechanisms by which casticin inhibits cell migration and invasion of mouse melanoma B16F10 cells. Cell viability was examined by MTT assay and the results indicated that casticin decreased the total percentages of viable cells in dose-dependent manners. Casticin affected cell migration and invasion in B16F10 cells were examined by wound healing mobility assay and Boyden chamber migration and invasion assay and results indicated that casticin inhibited cell migration and invasion in dose-dependent manners. Western blotting was used to examine the protein expression of B16F10 cells after exposed to casticin and the results showed that casticin decreased the expressions of MMP-9, MMP-2, MMP-1, FAK, 14-3-3, GRB2, Akt, NF-κB p65, SOS-1, p-EGFR, p-JNK 1/2, uPA, and Rho A in B16F10 cells. Furthermore, cDNA microarray assay was used to show that casticin affected associated gene expression of cell migration and invasion and the results indicated that casticin affected some of the gene expression such as increased SCN1B (cell adhesion molecule 1) and TIMP2 (TIMP metallopeptidase inhibitor 2) and decreased NDUFS4 (NADH dehydrogenase (ubiquinone) Fe-S protein4), VEGFA (vascular endothelial growth factor A), and DDIT3 (DNA-damage-inducible transcript 3) which associated cell migration and invasion in B16F10 cells. Based on those observations, we suggest that casticin could be used as a novel anticancer metastasis of melanoma cancer in the future. |
| Databáze: | OpenAIRE |
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