Chronic dietary exposure to branched chain amino acids impairs glucose disposal in vegans but not in omnivores
Autor: | Vladimir Stich, Michal Anděl, J Tůmová, Jan Gojda, Jan Trnka, Moustafa Elkalaf, Eva Krauzová, Jana Potockova, Petr Waldauf, Radka Straková, Martin Jaček, Lenka Rossmeislová, Petr Tůma |
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Rok vydání: | 2016 |
Předmět: |
0301 basic medicine
Vitamin Adult Blood Glucose Male medicine.medical_specialty Diet Vegan Calorie Arginine medicine.medical_treatment Respiratory chain Medicine (miscellaneous) Adipose tissue 030209 endocrinology & metabolism Dietary Exposure 03 medical and health sciences chemistry.chemical_compound Young Adult 0302 clinical medicine Internal medicine Surveys and Questionnaires Medicine Humans Insulin Food science Prospective Studies Muscle Skeletal Exercise Vegans Nutrition and Dietetics Anthropometry Dose-Response Relationship Drug business.industry Metabolism Diet 030104 developmental biology Endocrinology chemistry Basal (medicine) Glucose Clamp Technique Female Dietary Proteins Insulin Resistance business Amino Acids Branched-Chain |
Zdroj: | European journal of clinical nutrition. 71(5) |
ISSN: | 1476-5640 |
Popis: | Branched chain amino acids (BCAA) are among nutrients strongly linked with insulin sensitivity (IS) measures. We investigated the effects of a chronic increase of BCAA intake on IS in two groups of healthy subjects differing in their basal consumption of BCAA, that is, vegans and omnivores. Eight vegans and eight matched omnivores (five men and three women in each group) received 15 g (women) or 20 g (men) of BCAA daily for 3 months. Anthropometry, blood analyses, glucose clamp, arginine test, subcutaneous abdominal adipose tissue (AT) and skeletal muscle (SM) biopsies (mRNA levels of selected metabolic markers, respiratory chain (RC) activity) were performed at baseline, after the intervention and after a 6 month wash-out period. Compared with omnivores, vegans had higher IS at baseline (GIR, glucose infusion rate: 9.6±2.4 vs 7.1±2.4 mg/kg/min, 95% CI for difference: 0.55 to 5.82) that declined after the intervention and returned to baseline values after the wash-out period (changes in GIR with 95% CI, 3–0 months: −1.64 [−2.5; −0.75] and 9-3 months: 1.65 [0.75; 2.54] mg/kg/min). No such change was observed in omnivores. In omnivores the intervention led to an increased expression of lipogenic genes (DGAT2, FASN, PPARγ, SCD1) in AT. SM RC activity increased in both groups. Negative impact of increased BCAA intake on IS was only detected in vegans, that is, subjects with low basal amino acids/BCAA intake, which appear to be unable to induce sufficient compensatory changes within AT and SM on a BCAA challenge. |
Databáze: | OpenAIRE |
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