Fluorescence lifetime imaging with time-gated detection of hyaluronidase using a long lifetime azadioxatriangulenium (ADOTA) fluorophore

Autor:	Julian Borejdo, Sebastian Requena, Rahul Chib, Zygmunt Gryczynski, Ignacy Gryczynski, Yuri M. Strzhemechny, Rafal Fudala, Mark E. Mummert
Rok vydání:	2017
Předmět:	0301 basic medicine Male Fluorescence-lifetime imaging microscopy Fluorophore Hyaluronoglucosaminidase 02 engineering and technology Fluorescence 03 medical and health sciences chemistry.chemical_compound Hyaluronidase Cell Line Tumor Hyaluronic acid medicine Fluorescence Resonance Energy Transfer Humans General Materials Science Hyaluronic Acid Instrumentation Spectroscopy Fluorescent Dyes Aza Compounds Ionophores Optical Imaging 021001 nanoscience & nanotechnology Atomic and Molecular Physics and Optics 030104 developmental biology Spectrometry Fluorescence chemistry Microscopy Fluorescence Biophysics 0210 nano-technology medicine.drug
Zdroj:	Methods and applications in fluorescence. 4(4)
ISSN:	2050-6120
Popis:	A fluorescence lifetime imaging probe with a long lifetime was used in combination with time-gating for the detection of hyaluronidase using hyaluronic acid as the probe template. This probe was developed by heavily labeling hyaluronic acid with long lifetime azadioxatriangulenium fluorophores (ADOTA). We used this probe to image hyaluronidase produced by DU-145 prostate cancer cells.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::71cda623901cf14e019d37e08f0ffca1 https://pubmed.ncbi.nlm.nih.gov/28192308 Zobrazit plný text záznamu