Growth differentiation factor-11 supplementation improves survival and promotes recovery after ischemic stroke in aged mice
| Autor: | Anjali Chauhan, Bhanu P. Ganesh, Louise D. McCullough, Robia G. Pautler, Diego Morales, Liang Zhu, Jian-Jun Jiang, Songmi Lee, Sunil A Sheth, Julia Kofler, Eric C. Mohan, Jacob Hudobenko |
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| Rok vydání: | 2020 |
| Předmět: |
Male
Aging medicine.medical_specialty Angiogenesis Blotting Western Enzyme-Linked Immunosorbent Assay White matter integrity Corpus callosum White matter Mice Cerebrospinal fluid Atrophy Internal medicine Animals Medicine Stroke Ischemic Stroke business.industry Brain Recovery of Function Cell Biology Human brain medicine.disease stroke Growth Differentiation Factors Disease Models Animal gliosis medicine.anatomical_structure Endocrinology Gliosis GDF11 Bone Morphogenetic Proteins Dietary Supplements medicine.symptom business Research Paper |
| Zdroj: | Aging (Albany NY) |
| ISSN: | 1945-4589 |
| Popis: | Growth differentiation factor (GDF) 11 levels decline with aging. The age-related loss of GDF 11 has been implicated in the pathogenesis of a variety of age-related diseases. GDF11 supplementation reversed cardiac hypertrophy, bone loss, and pulmonary dysfunction in old mice, suggesting that GDF11 has a rejuvenating effect. Less is known about the potential of GDF11 to improve recovery after an acute injury, such as stroke, in aged mice. GDF11/8 levels were assessed in young and aged male mice and in postmortem human brain samples. Aged mice were subjected to a transient middle cerebral artery occlusion (MCAo). Five days after MCAo, mice received and bromodeoxyuridine / 5-Bromo-2'-deoxyuridine (BrdU) and either recombinant GDF11 or vehicle for five days and were assessed for recovery for one month following stroke. MRI was used to determine cerebrospinal fluid (CSF) volume, corpus callosum (CC) area, and brain atrophy at 30 days post-stroke. Immunohistochemistry was used to assess gliosis, neurogenesis, angiogenesis and synaptic density. Lower GDF11/8 levels were found with age in both mice and humans (p |
| Databáze: | OpenAIRE |
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