Basophils help establish protective immunity induced by irradiated larval vaccination for filariasis
| Autor: | Marina N. Torrero, Edward Mitre, C. Paul Morris, Hajime Karasuyama, Ellen Mueller Fox, Blima K. Mitre, Marc P. Hübner |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
CD4-Positive T-Lymphocytes
Antibodies Helminth chemical and pharmacologic phenomena Basophil Article Lymphocyte Depletion Filariasis Interferon-gamma Mice Immune system Antigen parasitic diseases medicine Helminths Animals Mast Cells Filarioidea Immunity Cellular Mice Inbred BALB C Vaccines General Veterinary General Immunology and Microbiology biology Interleukins Public Health Environmental and Occupational Health Antibodies Monoclonal hemic and immune systems Immunoglobulin E medicine.disease Mast cell Basophils Vaccination Infectious Diseases medicine.anatomical_structure Larva Immunology biology.protein Molecular Medicine Female Antibody |
| Popis: | Basophils are increasingly recognized as playing important roles in the immune response toward helminths. In this study, we evaluated the role of basophils in vaccine-mediated protection against filariae, tissue-invasive parasitic nematodes responsible for diseases such as elephantiasis and river blindness. Protective immunity and immunological responses were assessed in BALB/c mice vaccinated with irradiated L3 stage larvae and depleted of basophils with weekly injections of anti-CD200R3 antibody. Depletion of basophils after administration of the vaccination regimen but before challenge infection did not alter protective immunity. In contrast, basophil depletion initiated prior to vaccination and continued after challenge infection significantly attenuated the protective effect conferred by vaccination. Vaccine-induced cellular immune responses to parasite antigen were substantially decreased in basophil-depleted mice, with significant decreases in CD4(+) T-cell production of IL-4, IL-5, IL-10, and IFN-γ. Interestingly, skin mast cell numbers, which increased significantly after vaccination with irradiated L3 larvae, were unchanged after vaccination in basophil-depleted mice. These findings demonstrate that basophils help establish the immune responses responsible for irradiated L3 vaccine protection. |
| Databáze: | OpenAIRE |
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