Insights into the leaves of Ceriscoides campanulata: Natural proanthocyanidins alleviate diabetes, inflammation, and esophageal squamous cell cancer via in vitro and in silico models
| Autor: | Md. Josim Uddin, Immacolata Faraone, Md. Anwarul Haque, Md. Mahbubur Rahman, Mohammad A. Halim, Frank D. Sönnichsen, Serhat Sezai Çiçek, Luigi Milella, Christian Zidorn |
|---|---|
| Rok vydání: | 2022 |
| Předmět: |
Pharmacology
Inflammation Mammals Esophageal Neoplasms Molecular Structure Epithelial Cells Rubiaceae alpha-Glucosidases General Medicine Molecular Docking Simulation Plant Leaves Butyrylcholinesterase Drug Discovery Acetylcholinesterase Carcinoma Squamous Cell Diabetes Mellitus Animals Humans Computer Simulation Glycoside Hydrolase Inhibitors Proanthocyanidins alpha-Amylases |
| Zdroj: | Fitoterapia. 158 |
| ISSN: | 1873-6971 |
| Popis: | Fourteen flavones (1-14) including twelve polymethoxylated flavones, two A-type proanthocyanidins (oligomeric flavonoids) (15, 16), one benzoyl glucoside (17), one triterpenoid (18), and one phenylpropanoid (19) were isolated from the leaves of the South Asian medicinal plant Ceriscoides campanulata (Roxb.) Tirveng (Rubiaceae). The structures of the compounds were identified based on their spectroscopic and spectrometric data and in comparison with literature data. Isolated compounds were tested in vitro against inflammatory enzymes (COX-2, iNOS), pro-inflammatory cytokines (IL-1β, IL-6, TNF-α), esophageal squamous carcinoma cell line (TE13), and carbohydrate digestion enzymes (α-amylase, α-glucosidase). Proanthocyanidins 15 and 16 significantly attenuated the LPS-induced inflammatory response of COX-2, iNOS, IL-1β, IL-6, TNF-α in RAW 264.7 cells. Proanthocyanidins also satisfactorily inhibited the regrowth (64%), migration (51%), and formation of tumor-spheres (48%) in ESCC cell line TE13 at 50% toxic concentration. Compounds 15 and 16 showed the most potent effect against mammalian α-amylase (IC |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect