Primer design for complete sequencing of the Avastrovirus ORF2 gene
| Autor: | Fábio Gregori, Luis Ramiro Luna Espinoza, Laila A. R. Beserra, Rodrigo Martins Soares |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Avastrovirus
Hepatitis Avian GENÉTICA MICROBIANA viruses Disease epidemiology virus diseases Avian nephritis virus Biology medicine.disease lcsh:S1-972 Virology Virus Orf2 gene PCR medicine Sequencing ORF2 lcsh:Agriculture (General) Primer (molecular biology) General Agricultural and Biological Sciences Nephritis |
| Zdroj: | Semina: Ciências Agrárias, Vol 39, Iss 1, Pp 411-418 (2018) Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| ISSN: | 1679-0359 1676-546X |
| DOI: | 10.5433/1679-0359.2018v39n1p411 |
| Popis: | Avastrovirus infection is associated with enteric disease, nephritis, and hepatitis in birds. In this study, we present a protocol for the complete sequencing of the ORF2 gene of avian nephritis virus (ANV), chicken astrovirus (CAstV), and turkey astrovirus type 1 (TAstV-1) using a conventional Sanger technique. Previously and newly designed primer pairs targeting both the conserved flanking and internal regions of the ORF2 gene of these three viruses were used. The information derived from the astroviral sequences obtained in this study is fundamental for characterizing this virus and providing data regarding several aspects of disease epidemiology and prevention. |
| Databáze: | OpenAIRE |
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