Cloning and expression of mouse integrin beta p(beta 7): a functional role in Peyer's patch-specific lymphocyte homing
| Autor: | David T. Crowe, Mickey C.T. Hu, Bernhard Holzmann, Irving L. Weissman |
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| Rok vydání: | 1992 |
| Předmět: |
Integrins
Blotting Western Molecular Sequence Data Integrin High endothelial venules Receptors Lymphocyte Homing Fluorescent Antibody Technique Gene Expression Mice Peyer's Patches Cell Adhesion medicine Animals Amino Acid Sequence RNA Messenger Cloning Molecular Cell adhesion Lymphocyte homing receptor Multidisciplinary Base Sequence biology Cell adhesion molecule Antibodies Monoclonal Peyer's patch DNA Molecular biology Peyer Patch medicine.anatomical_structure biology.protein Integrin beta 6 Endothelium Vascular Research Article |
| Zdroj: | Proceedings of the National Academy of Sciences. 89:8254-8258 |
| ISSN: | 1091-6490 0027-8424 |
| DOI: | 10.1073/pnas.89.17.8254 |
| Popis: | Lymphocytes express integrin receptors, termed lymphocyte Peyer's patch high endothelial venule (HEV) adhesion molecules (LPAMs), that mediate their organ-specific adhesion to specialized HEVs found in mucosal lymphoid organs (Peyer's patches). LPAM-1 consists of a murine integrin alpha 4 noncovalently associated with integrin beta p. Here, we describe the cloning and expression of a mouse cDNA encoding beta p, which is an 806-amino acid transmembrane glycoprotein. The genomic Southern blot analysis indicates that beta p is the murine homologue of human beta 7. The function of alpha 4 beta 7 as a Peyer's patch-specific adhesion molecule was tested directly by expression of the murine beta 7 cDNA in an alpha 4+ beta 7-B-cell line or coexpression of the alpha 4 and beta 7 cDNAs in an alpha 4-beta 7-T-cell line. The transfected cells exhibited a new Peyer's patch-specific adhesive phenotype that could be specifically blocked by monoclonal antibodies against alpha 4 and beta 7. Moreover, an anti-beta 7 monoclonal antibody specifically blocked binding of normal lymphocytes to Peyer's patch HEV but did not inhibit their binding to peripheral lymph node HEVs, indicating that beta 7 is a unique component of the Peyer's patch-specific homing receptor. |
| Databáze: | OpenAIRE |
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