Improved photodynamic activity of porphyrin loaded into nanoparticles: an in vivo evaluation using chick embryos

Autor: B. Pegaz, Jean-Pierre Ballini, Elodie Debefve, Yvette Konan-Kouakou, Hubert van den Bergh, Florence Delie, Norbert Lange, Angelica Vargas, Robert Gurny
Rok vydání: 2004
Předmět:
Polymers
Photodynamic therapy (PDT)
medicine.medical_treatment
Drug Evaluation
Preclinical
Pharmaceutical Science
Photodynamic therapy
Chick Embryo
Chorioallantoic Membrane
chemistry.chemical_compound
Polylactic Acid-Polyglycolic Acid Copolymer
polycyclic compounds
Nanotechnology
Medicine
media_common
ddc:615
Biodegradable nanoparticles
Photosensitizing Agents
Drug Synergism
Drug Combinations
Pharmaceutical Solutions
PLGA
Chorioallantoic membrane
Lipophilicity
Hydrophobic and Hydrophilic Interactions
Switzerland
Drug
Porphyrins
Stereochemistry
media_common.quotation_subject
Drug delivery system
Fluorescence
Injections
Pharmacokinetics
In vivo
Animals
Lactic Acid
Particle Size
Photomedicine group
Dose-Response Relationship
Drug
Ethanol
business.industry
Chick chorioallantoic membrane (CAM) model
Porphyrin
Photochemotherapy
Solubility
chemistry
Biophysics
business
Chickens
Extravasation
Polyglycolic Acid
Porphyrin (p-THPP)
Extravasation of Diagnostic and Therapeutic Materials
Zdroj: International Journal of Pharmaceutics, Vol. 286, No 1-2 (2004) pp. 131-145
ISSN: 0378-5173
Popis: Hydrophobic porphyrins are potentially interesting molecules for the photodynamic therapy (PDT) of solid cancers or ocular vascularization diseases. Their pharmaceutical development is, however, hampered by their lipophilicity, which renders formulation difficult especially when intravenous administration is needed. Encapsulation of a lipophilic derivative of porphyrin, the meso-tetra(p-hydroxyphenyl)porphyrin (p-THPP), into polymeric biodegradable poly(D,L-lactide-co-glycolide) (PLGA) nanoparticles proved to enhance its photodynamic activity against mammary tumour cells when compared to free drug. In order to further investigate these carriers, the efficacy of the encapsulated drug was assessed on the chick embryo chorioallantoic membrane (CAM) model. First, we identified a suitable solvent for the drug in terms of p-THPP solubility and tolerability by chick embryos. This solution was used as a reference. Then, the fluorescence pharmacokinetics and the photodynamic effects of the porphyrin on CAM vessels were evaluated after intravenous administration of either a p-THPP solution (free drug) or the drug loaded into nanoparticles. The results showed that: (i) the drug remained longer in the vascular compartment when incorporated into nanoparticles and (ii) vascular effects of p-THPP after light irradiation were enhanced with nanoparticle carriers. These results are discussed taking into account the extravasation of intravascular circulating photosensitizers and its influence on PDT performance.
Databáze: OpenAIRE
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