OsCERK1 plays a crucial role in the lipopolysaccharide-induced immune response of rice
| Autor: | Yusuke Kouzai, Keito Seko, Yoshitake Desaki, Antonio Molinaro, Yusuke Ninomiya, Naoto Shibuya, Yoko Nishizawa, Hanae Kaku, Yumi Shimizu, Eiichi Minami, Ryosuke Iwase |
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| Přispěvatelé: | Desaki, Yoshitake, Kouzai, Yusuke, Ninomiya, Yusuke, Iwase, Ryosuke, Shimizu, Yumi, Seko, Keito, Molinaro, Antonio, Minami, Eiichi, Shibuya, Naoto, Kaku, Hanae, Nishizawa, Yoko |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Lipopolysaccharides Lipopolysaccharide Arabidopsis thaliana Physiology Mutant Arabidopsis Plant Science Biology chitin immune response 03 medical and health sciences chemistry.chemical_compound Gene Expression Regulation Plant Plant Cells Gene expression Plant Immunity Receptor MAMP Plant Proteins OsCERK1 fungi food and beverages Oryza biology.organism_classification Cell biology microbe-associated molecular patterns (MAMPs) Oryza sativa (rice) 030104 developmental biology chemistry Mutation Peptidoglycan Bacterial outer membrane Reactive Oxygen Species lipopolysaccharide (LPS) |
| Zdroj: | SC30201804040061 NARO成果DBa |
| DOI: | 10.1111/nph.14941 |
| Popis: | Plant cell surface receptor-like kinases (RLKs) mediate the signals from microbe-associated molecular patterns (MAMPs) that induce immune responses. Lipopolysaccharide (LPS), the major constituent of the outer membrane of gram-negative bacteria, is a common MAMP perceived by animals and plants; however, the plant receptors/co-receptors are unknown except for LORE, a bulb-type lectin S-domain RLK (B-lectin SD1-RLK) in Arabidopsis. OsCERK1 is a multifunctional RLK in rice that contains lysin motifs (LysMs) and is essential for the perception of chitin, a fungal MAMP, and peptidoglycan, a bacterial MAMP. Here, we analyzed the relevance of OsCERK1 to LPS perception in rice. Using OsCERK1-knockout mutants (oscerk1), we evaluated hydrogen peroxide (H2 O2 ) production and gene expression after LPS treatment. We also examined the LPS response in knockout mutants for the B-lectin SD1-RLK genes in rice and for all LysM-protein genes in Arabidopsis. Compared with wild-type rice cells, LPS responses in oscerk1 cells were mostly diminished. By contrast, rice lines mutated in either of three B-lectin SD1-RLK genes and Arabidopsis lines mutated in the LysM-protein genes responded normally to LPS. From these results, we conclude that OsCERK1 is an LPS receptor/co-receptor and that the LPS perception systems of rice and Arabidopsis are significantly different. |
| Databáze: | OpenAIRE |
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