Effect of tamoxifen on fibrosis, collagen content and transforming growth factor-β1, -β2 and -β3 expression in common bile duct anastomosis of pigs

Autor:	Beni Olej, Antonio Claudio Lucas da Nóbrega, Karen Jesus Oliveira, Bruno Felix-Patrício, Orlando Hiroshi Kiono Siqueira, Angela Cristina Gouvêa Carvalho, Fábio Otero Ascoli, Renata Frauches Medeiros
Rok vydání:	2017
Předmět:	Male medicine.medical_specialty Sus scrofa Drug Evaluation Preclinical 030230 surgery Anastomosis Pathology and Forensic Medicine Picrosirius red Masson's trichrome stain Transforming Growth Factor beta1 03 medical and health sciences Transforming Growth Factor beta2 0302 clinical medicine Transforming Growth Factor beta3 Fibrosis Transforming Growth Factor beta Internal medicine Medicine Animals RNA Messenger Molecular Biology Common Bile Duct Wound Healing Common bile duct business.industry Bile duct Anastomosis Surgical Cell Biology Original Articles medicine.disease Tamoxifen medicine.anatomical_structure Endocrinology 030220 oncology & carcinogenesis Collagen business medicine.drug Transforming growth factor
Zdroj:	International journal of experimental pathology. 98(5)
ISSN:	1365-2613
Popis:	End-to-end anastomosis in the treatment for bile duct injury during laparoscopic cholecystectomy has been associated with stricture formation. The aim of this study was to experimentally investigate the effect of oral tamoxifen (tmx) treatment on fibrosis, collagen content and transforming growth factor-β1, -β2 and -β3 expression in common bile duct anastomosis of pigs. Twenty-six pigs were divided into three groups [sham (n = 8), control (n = 9) and tmx (n = 9)]. The common bile ducts were transected and anastomosed in the control and tmx groups. Tmx (40 mg/day) was administered orally to the tmx group, and the animals were euthanized after 60 days. Fibrosis was analysed by Masson's trichrome staining. Picrosirius red was used to quantify the total collagen content and collagen type I/III ratio. mRNA expression of transforming growth factor (TGF)-β1, -β2 and -β3 was quantified using real-time polymerase chain reaction (qRT-PCR). The control and study groups exhibited higher fibrosis than the sham group, and the study group showed lower fibrosis than the control group (P = 0.011). The control and tmx groups had higher total collagen content than the sham group (P = 0.003). The collagen type I/III ratio was higher in the control group than in the sham and tmx groups (P = 0.015). There were no significant differences in the mRNA expression of TGF-β1, -β2 and -β3 among the groups (P > 0.05). Tmx decreased fibrosis and prevented the change in collagen type I/III ratio caused by the procedure.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::71ae0cac36ec38fc0d929f1085cd6bbc https://pubmed.ncbi.nlm.nih.gov/29205609 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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