Optimization of the photodynamic inactivation of prions by a phthalocyanine photosensitizer: The crucial involvement of singlet oxygen
| Autor: | Jaroslav Freisleben, Zdenka Backovska Hanusova, Jiri Mosinger, Ales Hamacek, Daniela Moravcova, Marie Kostelanska, Tibor Mosko, Karel Holada, R. Vik |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Indoles
General Physics and Astronomy Isoindoles phthalocyanines 01 natural sciences Brain homogenate Prion Proteins General Biochemistry Genetics and Molecular Biology singlet oxygen photodynamic inactivation 010309 optics Protein content Mice chemistry.chemical_compound 0103 physical sciences Animals General Materials Science Photosensitizer Red light Prion protein prions Photosensitizing Agents Singlet oxygen 010401 analytical chemistry General Engineering Brain General Chemistry decontamination 0104 chemical sciences nervous system diseases Light intensity Photochemotherapy chemistry Biophysics Phthalocyanine Sulfonic Acids |
| Popis: | Prion disorders are fatal neurodegenerative diseases caused by the autocatalytic conversion of a natively occurring prion protein (PrPC ) into its misfolded infectious form (PrPTSE ). The proven resistance of PrPTSE to common disinfection procedures increases the risk of prion transmission in medical settings. Herein, we present the effective photodynamic inactivation (PDI) of prions by disulfonated hydroxyaluminum phthalocyanine (AlPcOH(SO3 )2 ) utilizing two custom-built red light sources. The treatment eliminates PrPTSE signal in infectious mouse brain homogenate with efficiency that depends on light intensity but has a low effect on the overall protein content. Importantly, singlet oxygen (O2 (1 Δg )) is the only species significantly photogenerated by AlPcOH(SO3 )2 , and it is responsible for the PDI of prions. More intensive light conditions show not only higher O2 (1 Δg ) production but also decreases in AlPcOH(SO3 )2 photostability. Our findings suggest that PDI by AlPcOH(SO3 )2 -generated O2 (1 Δg ) represents a promising approach for prion inactivation that may be useful in future decontamination strategies for delicate medical tools. |
| Databáze: | OpenAIRE |
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