Improved 2,3-butanediol yield and productivity from lignocellulose biomass hydrolysate in metabolically engineered Enterobacter aerogenes
| Autor: | Ja Kyong Ko, Seok Woo Yoo, Min Kyu Oh, Minsun Kim, Youngsoon Um, Duck Gyun Kim |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
Environmental Engineering Lignocellulosic biomass Bioengineering 010501 environmental sciences Xylose Enterobacter aerogenes Lignin 01 natural sciences Hydrolysate Metabolic engineering chemistry.chemical_compound 010608 biotechnology 2 3-Butanediol Biomass Food science Butylene Glycols Waste Management and Disposal 0105 earth and related environmental sciences biology Renewable Energy Sustainability and the Environment Chemistry General Medicine biology.organism_classification Glucose Metabolic Engineering Butanediol Fermentation |
| Zdroj: | Bioresource Technology. 309:123386 |
| ISSN: | 0960-8524 |
| Popis: | We previously engineered Enterobacter aerogenesfor glucose and xylose co-utilization and 2,3-butanediol production. Here, strain EMY-22 was further engineered to improve the 2,3-butanediol titer, productivity, and yield by reducing the production of byproducts. To reduce succinate production, the budABC operon and galP gene were overexpressed, which increased 2,3-butanediol production. For further reduction of succinate and 2-ketogluconate production, maeA was selected and overexpressed in EMY-22. The optimally engineered strain produced 2,3-butanediol for a longer time and showed reduced byproduct formation from sugarcane bagasse hydrolysate under flask cultivation conditions. The engineered strain displayed 66.6, 13.4, and 16.8% improvements in titer, yield, productivity of 2,3-butanediol, respectively, compared to its parental strain under fed-batch fermentation conditions. The data demonstrate that the metabolic engineering to reduce byproduct formation is a promising strategy to improve 2,3-butanediol production from lignocellulosic biomass. |
| Databáze: | OpenAIRE |
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