The effects of different syringe volume, needle size and sample volume on blood gas analysis in syringes washed with heparin
| Autor: | Tuncay Küme, Ali Riza Sisman, Birsen Tuğlu, Ahmet Solak, Burcu Çinkooğlu, Canan Çoker |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
medicine.medical_specialty
Point-of-Care Systems Clinical Biochemistry blood gas analysis blood specimen collection heparin needles syringes Chemistry Techniques Analytical Sample volume Electrolytes medicine Humans Diagnostic Errors Syringe Blood gas analysis Blood Specimen Collection Chromatography Heparin Biochemistry (medical) Equipment Design Hydrogen-Ion Concentration Original Papers Dilution Surgery Needle size Volume (thermodynamics) Calibration medicine.drug |
| Zdroj: | Biochemia Medica Volume 22 Issue 2 |
| ISSN: | 1846-7482 1330-0962 |
| Popis: | Introduction: We evaluated the eff ect of diff erent syringe volume, needle size and sample volume on blood gas analysis in syringes washed w ith heparin. Materials and methods: In this multi-step experimental study, percent dilution ratios (PDRs) and fiheparin concentrations (FHCs) were calcu- lated by gravimetric method for determining the eff ect of syringe volume (1, 2, 5 and 10 mL), needle size (20, 21, 22, 25 and 2 6 G) and sample volu- me (0.5, 1, 2, 5 and 10 mL). The eff ect of diff erent PDRs and FHCs on blood gas and electrolyte parameters were determined. The erroneous results from nonstandardized sampling were evaluated according to RiliBAK's TEa. Results: The increase of PDRs and FHCs was associated with the decrease of syringe volume, the increase of needle size and the decrease of sample volume: from 2.0% and 100 IU/mL in 10 mL-syringe to 7.0% and 351 IU/mL in 1 mL-syringe; from 4.9% and 245 IU/mL in 26G to 7.6% and 380 IU/mL in 20 G with combined 1 mL syringe; from 2.0% and 100 IU/mL in full-fi lled sample to 34% and 1675 IU/mL in 0.5 mL suctioned sam ple into 10 mL- syringe. There was no statistical diff erence in pH; but the percent decreasing in pCO 2 , K + , iCa 2+ , iMg 2+ ; the percent increasing in pO 2 and Na + were statistical signifi cance compared to samples full-fi lled in syringes. The all changes in pH and pO 2 were acceptable; but the changes in pCO 2 , Na + , K + and iCa 2+ were unacceptable according to TEa limits except fullfi lled-syringes. Conclusions: The changes in PDRs and FHCs due nonstandardized sampling in syringe washed with liquid heparin give rise to erroneous test results for pCO 2 and electrolytes. |
| Databáze: | OpenAIRE |
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