Investigation of 1377C/T polymorphism of the Toll-like receptor 3 among patients with chronic hepatitis B
| Autor: | Cemal Bulut, Ali Pekcan Demiröz, Emine Fırat Göktaş, Erdem Kamil Ozer, Atilla Bozkurt, Ragip Ozgur Karaca, Sami Kinikli, Mustafa Tugrul Goktas |
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| Přispěvatelé: | Farmakoloji, Selçuk Üniversitesi |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Adult Male Biochemistry & Molecular Biology Hepatitis B virus Immunology Single-nucleotide polymorphism Biology medicine.disease_cause Applied Microbiology and Biotechnology Microbiology Polymorphism Single Nucleotide Pathogenesis 03 medical and health sciences Hepatitis B Chronic Genotype Genetics medicine Humans chronic hepatitis B Receptor Molecular Biology Gene Aged General Medicine single-nucleotide polymorphism Hepatitis B Middle Aged medicine.disease Toll-like receptors Toll-Like Receptor 3 030104 developmental biology Biotechnology & Applied Microbiology Female Restriction fragment length polymorphism Polymorphism Restriction Fragment Length |
| Zdroj: | Canadian journal of microbiology. 62(7) |
| ISSN: | 1480-3275 |
| Popis: | WOS: 000378785100009 PubMed: 27314608 The immunopathogenesis of chronic hepatitis B (CHB) has not been clarified yet. Toll-like receptors (TLR) are a receptor family that initiates immunity with exogenous-endogenous ligands and plays a role in the pathogenesis of infections. In this study, we aimed to investigate the frequency of TLR 3 1377C/T (rs3775290) polymorphism and its role in patients with CHB. We included 50 healthy individuals as control group and 73 active and 43 inactive hepatitis B patients. All DNA samples were isolated from blood samples. For the detection of TLR 3 1377C/T single-nucleotide polymorphism, restriction fragment length polymorphism was used. A statistically significant difference was determined in Hepatitis B virus (HBV) DNA levels of CHB patients with the CC, CT, and TT genotypes (p = 0.013). The highest levels of HBV DNA were detected in individuals with TT genotypes. Additionally, the frequency of CC genotype was higher in the active CHB patients compared with that of the inactive CHB patients (p = 0.044). No statistically significant difference in TLR 3 1377C/T polymorphism was detected between healthy controls and the hepatitis B patients (p = 0.342). In conclusion, HBV DNA level was higher in the individuals with TT genotype, and CC genotype was more frequent in the active CHB patients. These results suggest a possible association between CHB and TLR 3 gene (1377C/T) polymorphism. |
| Databáze: | OpenAIRE |
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