Ultraviolet B irradiation increases the expression of inflammatory cytokines in cultured sebocytes
| Autor: | Do Won Kim, Seok-Jong Lee, Weon Ju Lee, Mi Yeung Sohn, Kyung Hea Park, Won Chae Lee |
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| Rok vydání: | 2013 |
| Předmět: |
medicine.medical_specialty
Ultraviolet Rays Lymphokine Interleukin Dermatology General Medicine Biology Molecular biology In vitro Proinflammatory cytokine Reverse transcription polymerase chain reaction Sebaceous Glands Endocrinology Internal medicine Acne Vulgaris Gene expression medicine Cytokines Humans Tumor necrosis factor alpha Interleukin 8 Inflammation Mediators Cells Cultured |
| Zdroj: | The Journal of Dermatology. 40:993-997 |
| ISSN: | 0385-2407 |
| DOI: | 10.1111/1346-8138.12330 |
| Popis: | Sebaceous gland hyperplasia and increased sebum secretion after irradiation of ultraviolet (UV)-B has been widely accepted. This study was performed to clarify expression of inflammatory cytokines after irradiating UV-B in cultured sebocytes. Reverse transcription polymerase chain reaction was performed to measure gene expression of inflammatory cytokines, including interleukin (IL)-1β, IL-6, IL-8 and tumor necrosis factor-α, in cultured sebocytes after exposure to 40 and 70 mJ/cm(2) UV-B. Protein expression of inflammatory cytokines and lipid production in cultured sebocytes after exposure to UV-B were measured using enzyme-linked immunoassay and lipid analysis kit. The expression of inflammatory cytokines, especially IL-1β and IL-8, was significantly increased in cultured sebocytes after treatment with UV-B. Many more studies on the effect of UV-B on sebaceous glands should be performed to reveal the pathogenic mechanism of acne. |
| Databáze: | OpenAIRE |
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