Isolation and Short-Term Persistence ofEhrlichia ewingiiin Cell Culture
| Autor: | Lindsay F. Killmaster, Michael L. Levin |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Ehrlichia ewingii 030231 tropical medicine Ehrlichia Buffy coat Biology Microbiology Article Cell Line Amblyomma americanum 03 medical and health sciences 0302 clinical medicine Virology Animals Humans Pathogen Direct fluorescent antibody Bacteriological Techniques biology.organism_classification Culture Media 030104 developmental biology Infectious Diseases Cell culture Ehrlichiosis (canine) |
| Zdroj: | Vector-Borne and Zoonotic Diseases. 16:445-448 |
| ISSN: | 1557-7759 1530-3667 |
| DOI: | 10.1089/vbz.2015.1938 |
| Popis: | Ehrlichia ewingii is the causative agent of human and canine granulocytic ehrlichiosis. Since its discovery in 1970, little work has been done to characterize the pathogen or study the transmission dynamics due to the inability to grow the agent in vitro. The aim of this study was to assess the suitability of multiple cell lines and media formulations for propagation of E. ewingii in cell culture. In this study, we present an overview of attempts to isolate E. ewingii from the buffy coat of goats naturally infected by Amblyomma americanum ticks, as well as a methodology for maintaining the pathogen for up to 16 weeks in culture. The most promising results were seen with HL-60 cells differentiated by the addition of 1.5% DMSO to the media and supplemented with 8 mM l-glutamine. Cultures were passaged multiple times, and fluorescence and morulae were observed by indirect fluorescent antibody test and Diff-Quik staining. It is our hope that this information will provide a foundation for future attempts to propagate and maintain E. ewingii in cell culture. |
| Databáze: | OpenAIRE |
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