The β-adrenoceptor agonist isoproterenol promotes the activity of respiratory chain complex I and lowers cellular reactive oxygen species in fibroblasts and heart myoblasts
| Autor: | Consiglia Pacelli, Loris Micelli, Arcangela Santeramo, Tiziana Cocco, Francesco Papa, M Scivetti, Maria Larizza, Nicola Sardaro, Domenico De Rasmo, Giuliano Gattoni, Anna Signorile, Serena Milano |
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| Rok vydání: | 2011 |
| Předmět: |
Agonist
medicine.medical_specialty medicine.drug_class Respiratory chain Mitochondrion Biology chemistry.chemical_compound Isoprenaline Internal medicine Cyclic AMP medicine Humans Phosphorylation Fibroblast Cells Cultured Pharmacology chemistry.chemical_classification Reactive oxygen species Electron Transport Complex I Infant Newborn Isoproterenol Okadaic acid Adrenergic beta-Agonists Fibroblasts Mitochondria Endocrinology medicine.anatomical_structure chemistry Reactive Oxygen Species Myoblasts Cardiac medicine.drug |
| Zdroj: | European Journal of Pharmacology. 652:15-22 |
| ISSN: | 0014-2999 |
| DOI: | 10.1016/j.ejphar.2010.11.016 |
| Popis: | A study is presented on the effect of the β-adrenoceptor agonist isoproterenol on mitochondrial oxygen metabolism in fibroblast and heart myoblast cultures. Isoproterenol treatment of serum-limited fibroblasts and proliferating myoblasts results in the promotion of mitochondrial complex I activity and decrease of the cellular level of reactive oxygen species. These effects of isoproterenol are associated with cAMP-dependent phosphorylation of complex I subunit(s). Addition of okadaic acid, inhibitor of protein phosphatase(s), reverses the decline of complex I activity in serum-limited fibroblast cultures and activates the complex in proliferating myoblast cultures. The effects of isoproterenol on complex I activity and reactive oxygen species balance can contribute to the therapeutic effect of the drug. |
| Databáze: | OpenAIRE |
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