Transgenic Potato Plants Expressing Soybean ß-1,3-Endoglucanase Gene Exhibit an Increased Resistance to Phytophthora infestans
| Autor: | Malik F. M. Awan, Ludmila Yakovleva, Kazimierz Kleczkowski, Maria Borkowska, Andrzej Talarczyk, Magdalena Krzymowska, Bernard Wielgat |
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| Rok vydání: | 1998 |
| Předmět: |
Phytophthora
Base Sequence biology Agrobacterium beta-Glucosidase Transgene fungi food and beverages Glucan 1 3-beta-Glucosidase Genetically modified crops Agrobacterium tumefaciens Plants Genetically Modified biology.organism_classification General Biochemistry Genetics and Molecular Biology Microbiology Transformation (genetics) Complementary DNA Botany Phytophthora infestans Soybeans Gene DNA Primers Solanum tuberosum |
| Zdroj: | Zeitschrift für Naturforschung C. 53:1012-1016 |
| ISSN: | 1865-7125 0939-5075 |
| DOI: | 10.1515/znc-1998-11-1212 |
| Popis: | Soybean β-1,3-endoglucanase represents a model system for studies on early plant responses to infection by fungal pathogens, and it has been implicated in the release of elicitors from fungal cell walls. In the present study, potato plants were transformed with the soybean β-1,3-endoglucanase cDNA via Agrobacterium delivery system. The transfer of the gene into potato genome was confirmed by (i) PCR amplification, (ii) Northern blot analyses, and (Hi) an increase in the activity of β-1,3-endoglucanase in transgenic plants. The transformation resulted in an increased resistance of selected transgenic plants to infection by Phytophthora infestans, an important pathogen. |
| Databáze: | OpenAIRE |
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