Comparison of the Vitek GPS-TB Card With Disk Diffusion Testing for Predicting the Susceptibility of Enterococci to Vancomycin
| Autor: | Carla E. Woodmansee, C. Glen Mayhall, Barbara S. Reisner, Natalie Williams-Bouyer, Pamela S. Falk |
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| Rok vydání: | 1999 |
| Předmět: |
Susceptibility testing
Repeat testing Microbial Sensitivity Tests Pathology and Forensic Medicine Microbiology Diffusion Vancomycin medicine Humans Diffusion (business) Agar screen Etest Antibacterial agent biology General Medicine biochemical phenomena metabolism and nutrition bacterial infections and mycoses equipment and supplies biology.organism_classification Anti-Bacterial Agents Medical Laboratory Technology Enterococcus bacteria medicine.drug |
| Zdroj: | Archives of Pathology & Laboratory Medicine. 123:622-625 |
| ISSN: | 1543-2165 0003-9985 |
| DOI: | 10.5858/1999-123-0622-cotvgt |
| Popis: | Objective.—To compare the ability of the Vitek GPS-TB card with disk diffusion testing for determining the susceptibility of enterococci to vancomycin. Design.—Vitek susceptibility testing was performed using the GPS-TB card and software version R05.03. Disk diffusion susceptibility testing was performed according to National Committee for Clinical Laboratory Standards guidelines. When discrepancies occurred between the interpretation of Vitek and disk diffusion, both tests were repeated and the epsilometer test (E test) and agar screen containing 6 μg/mL vancomycin were performed. Results.—Of 415 isolates tested, 313 were susceptible to vancomycin and 97 were resistant to vancomycin by both test methods. Two isolates were intermediate by Vitek and resistant by disk diffusion, 2 were intermediate by Vitek and susceptible by disk diffusion, and 1 was susceptible by Vitek and intermediate by disk diffusion. All but 1 of these latter 5 isolates (intermediate by Vitek and susceptible by disk diffusion) were available for retesting. On repeat testing, the 2 isolates that were intermediate by Vitek and resistant by disk diffusion were resistant by both methods, the 1 isolate that was intermediate by Vitek and susceptible by disk diffusion was susceptible by both methods, and the isolate that was susceptible by Vitek and intermediate by disk diffusion was also susceptible by both methods. These results were confirmed by E test and agar screen. Conclusion.—We found the results of the GPS-TB card compared well with disk diffusion. However, isolates with intermediate results by Vitek should be retested using another method, such as the E test. |
| Databáze: | OpenAIRE |
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