Synthesis of a hybrid polymer-inorganic biomimetic support incorporating in situ pectinase from Aspergillus niger ATCC 9642
| Autor: | Débora de Oliveira, Eunice Valduga, Geciane Toniazzo, Rogério Marcos Dallago, Marcelo Luis Mignoni, Luciana Dornelles Venquiaruto, Cindy Elena Bustamante-Vargas |
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| Rok vydání: | 2014 |
| Předmět: |
food.ingredient
Calcium alginate Alginates Calcium oxalate Bioengineering Sodium oxalate Gelatin Fungal Proteins chemistry.chemical_compound food Glucuronic Acid Biomimetic Materials Pectinase biology Calcium Oxalate Hexuronic Acids Aspergillus niger General Medicine Mineralization (soil science) biology.organism_classification Enzymes Immobilized Polygalacturonase Biochemistry chemistry Industrial and production engineering Biotechnology Nuclear chemistry |
| Zdroj: | Bioprocess and biosystems engineering. 38(8) |
| ISSN: | 1615-7605 |
| Popis: | The hybrid alginate/gelatin/calcium oxalate (AGOCa) support was successfully synthesized through the biomimetic mineralization method for immobilization in situ of a pectinolytic extract from Aspergillus niger ATCC 9642 via entrapment technique. The efficiency of immobilization reached 72.7 %. Sodium oxalate buffer (100 mM, pH 5.5) was selected as adjuvant of the immobilization process by allowing the formation of a calcified shell around the calcium alginate capsule, significantly increasing the stability to storage, thermal and recycling of the enzymatic immobilized pectinolytic extract. The pH and temperature for maximum activity were from 5.0 to 6.0 and 60 to 80 °C, respectively. The new hybrid support can be a potential alternative to obtain immobilized pectinases with properties for advantageous industrial applications. |
| Databáze: | OpenAIRE |
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