Long-peptide therapeutic vaccination against CRPV-induced papillomas in HLA-A2.1 transgenic rabbits
| Autor: | Karla K. Balogh, Xuwen Peng, Neil D. Christensen, Jiafen Hu, Nancy M. Cladel, Lynn R. Budgeon |
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| Rok vydání: | 2014 |
| Předmět: |
T cell
Transgenic rabbit Immunology Microbiology Epitope Article CRPV HLA-A2.1 Immune system medicine Cytotoxic T cell biology lcsh:RM1-950 Public Health Environmental and Occupational Health Cottontail rabbit papillomavirus T-cell mediated immune response Therapeutic vaccine Virology 3. Good health Vaccination Infectious Diseases medicine.anatomical_structure lcsh:Therapeutics. Pharmacology biology.protein Peptide vaccine Antibody Long peptide |
| Zdroj: | Trials in Vaccinology, Vol 3, Iss C, Pp 134-142 (2014) |
| ISSN: | 1879-4378 |
| DOI: | 10.1016/j.trivac.2014.06.002 |
| Popis: | Long peptide immunization is a promising strategy to clear established tumors. In the current study, we investigated the therapeutic effect of a naturally existing long peptide that contained two HLA-A2.1 restricted epitopes (CRPVE1/149–157 and CRPVE1/161–169) from cottontail rabbit papillomavirus (CRPV) E1 using our CRPV/HLA-A2.1 transgenic rabbit model. A universal Tetanus Toxin helper motif (TT helper) was tagged at either the N-terminus or the carboxyl-terminus of this long peptide and designated as TT-E1 peptide and E1 peptide-TT, respectively. Four groups of HLA-A2.1 transgenic rabbits were infected with wild type CRPV DNA. Three weeks post-infection, the rabbits were immunized four times with TT-E1 peptide, E1 peptide only, E1 peptide-TT or TT-control peptide with two-week intervals between immunizations. Tumor outgrowth was monitored and recorded weekly. After the third booster immunization, tumors on two of the four E1 peptide-TT immunized rabbits began to shrink. One animal from this group was free of tumors at the termination of the study. The mean papilloma size of E1 peptide-TT immunized rabbits was significantly smaller when compared with that of the three other groups (P |
| Databáze: | OpenAIRE |
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