Physical training prior to myocardial infarction potentializes stem cell therapy, SDF-1/CXCR4 axis activation and inhibits the vasoconstrictor response in hypertensive rats
| Autor: | Maximiliano Isoppo Schaun, Maria Claudia Irigoyen, Thiago Rodrigues Peres, Lucinara Dadda Dias, Melissa Kristochek, Alexandre Machado Lehnen, Melissa Medeiros Markoski |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Receptors CXCR4 Cardiotonic Agents Angiogenesis medicine.medical_treatment Immunology Myocardial Infarction Biochemistry CXCR4 Ventricular Function Left Cell therapy 03 medical and health sciences 0302 clinical medicine Internal medicine Physical Conditioning Animal Rats Inbred SHR medicine Immunology and Allergy Animals cardiovascular diseases Vasoconstrictor Agents Myocardial infarction Molecular Biology Ejection fraction business.industry Stroke Volume Hematology Stem-cell therapy medicine.disease Chemokine CXCL12 Rats 030104 developmental biology Echocardiography Vasoconstriction 030220 oncology & carcinogenesis Hypertension Cardiology Female Stem cell Sedentary Behavior business Stem Cell Transplantation |
| Zdroj: | Cytokine. 126 |
| ISSN: | 1096-0023 |
| Popis: | Stem cell therapy is a promising strategy for recovering of injured cardiac tissue after acute myocardial infarction. The effects promoted by preventive physical training, beneficial for regeneration, are not yet understood on stem cell homing. In the present study, we evaluated the effect of preventive physical training on cell homing activation and associated mechanisms after acute myocardial infarction and therapy with adipose-derived stem cells in spontaneously hypertensive rats (SHR). Forty female SHR were allocated in sedentary (S), sedentary SHAM (S-SHAM), sedentary AMI (S-AMI), sedentary with cell therapy (S-ICT), aerobically trained (T), trained SHAM (T-SHAM), trained AMI (T-AMI) and trained with cell therapy (S-ICT) groups. Cell therapy was performed through the infusion of 2 × 105 ADSC/0.05 mL at the moment of AMI. Molecular markers of cell homing (SDF-1/CXCR4), inflammatory response (myeloperoxidase and cardiac expression of iNOS, gp91phox and NFkB), vasoconstrictor agents (Ang II and ET-1) and an angiogenesis inducer (VEGF) were measured. Functional capacity and echocardiographic parameters were also evaluated. Preventive physical training associated with cell therapy was able to reduce left ventricle ejection fraction losses in infarcted animals. Results demonstrated activation of the SDF-1/CXCR4 axis by physical training, besides a reduction in vasoconstrictor and systemic inflammatory responses. Physical training prior to AMI was able to induce a cardioprotective effect and optimize the reparative mechanism of cell therapy in an animal model of hypertension. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect