MIND model for triple-negative breast cancer in syngeneic mice for quick and sequential progression analysis of lung metastasis

Autor: Snigdha Banerjee, Stephanie Graff, Sushanta K. Banerjee, Ossama Tawfik, Sandipto Sarkar, Arnab Ghosh, Fariba Behbod, Douglas H. McGregor
Rok vydání: 2018
Předmět:
0301 basic medicine
Lung Neoplasms
lcsh:Medicine
Triple Negative Breast Neoplasms
Disease
Lung and Intrathoracic Tumors
Metastasis
Mesoderm
Mice
0302 clinical medicine
Basic Cancer Research
Breast Tumors
Medicine and Health Sciences
lcsh:Science
Triple-negative breast cancer
Staining
Multidisciplinary
Cell Staining
Animal Models
Mammary Glands
3. Good health
Cell Transformation
Neoplastic
Lymphatic system
Oncology
Experimental Organism Systems
030220 oncology & carcinogenesis
Disease Progression
Experimental pathology
Female
Anatomy
Research Article
Mouse Models
Research and Analysis Methods
03 medical and health sciences
Model Organisms
Exocrine Glands
Breast cancer
Cell Line
Tumor
Breast Cancer
medicine
Animals
Humans
Neoplasm Invasiveness
Cell Proliferation
Cell growth
business.industry
lcsh:R
Reproductive System
Cancers and Neoplasms
Biology and Life Sciences
Mammary Neoplasms
Experimental
medicine.disease
Disease Models
Animal
030104 developmental biology
Metastatic Tumors
Specimen Preparation and Treatment
Cell culture
Cancer research
lcsh:Q
Secondary Lung Tumors
business
Breast Tissue
Zdroj: PLoS ONE
PLoS ONE, Vol 13, Iss 5, p e0198143 (2018)
ISSN: 1932-6203
Popis: Mouse models of breast cancer with specific molecular subtypes (e.g., ER or HER2 positive) in an immunocompetent or an immunocompromised environment significantly contribute to our understanding of cancer biology, despite some limitations, and they give insight into targeted therapies. However, an ideal triple-negative breast cancer (TNBC) mouse model is lacking. What has been missing in the TNBC mouse model is a sequential progression of the disease in an essential native microenvironment. This notion inspired us to develop a TNBC-model in syngeneic mice using a mammary intraductal (MIND) method. To achieve this goal, Mvt-1and 4T1 TNBC mouse cell lines were injected into the mammary ducts via nipples of FVB/N mice and BALB/c wild-type immunocompetent mice, respectively. We established that the TNBC-MIND model in syngeneic mice could epitomize all breast cancer progression stages and metastasis into the lungs via lymphatic or hematogenous dissemination within four weeks. Collectively, the syngeneic mouse-TNBC-MIND model may serve as a unique platform for further investigation of the underlying mechanisms of TNBC growth and therapies.
Databáze: OpenAIRE
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