Safety evaluation, anti-oxidative and anti-inflammatory effects of subchronically dietary supplemented high dosing grape seed powder (GSP) to healthy rat
| Autor: | Mohamed Mahmoudi, Ezzedine Aouani, Khawla Jebari, Takwa Bedhiafi, Michèle Veronique El May, Kamel Charradi, Ferid Limam |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Male Antioxidant medicine.drug_class medicine.medical_treatment Neurogenesis Anti-Inflammatory Agents chemistry.chemical_element Pharmacology Calcium Weight Gain Hippocampus Anti-inflammatory Antioxidants 03 medical and health sciences chemistry.chemical_compound stomatognathic system medicine Animals Rats Wistar Creatinine Kidney Adiponectin Grape Seed Extract Dentate gyrus General Medicine Organ Size 030104 developmental biology medicine.anatomical_structure chemistry Organ Specificity Dietary Supplements Urea Lipid Peroxidation Powders |
| Zdroj: | Biomedicinepharmacotherapy = Biomedecinepharmacotherapie. 107 |
| ISSN: | 1950-6007 |
| Popis: | Grape seed powder (GSP) contains high amount of bioactive polyphenols usually used as nutritional supplement or food preservatives due to their antioxidant and scavenging properties. The purpose of the present work was to evaluate the safety of increasing dosage GSP (w/w) of 0.5%, 5%, 10% and 20% corresponding to 0.4, 4, 8 and 16 g/kg bw respectively, when administered sub-chronically to Wistar rats in a 2 month-repeated dosing oral toxicity trial. Overally GSP had no effect on food intake, decreased body weight gain without affecting brain, liver, heart or kidney relative weight. GSP did not alter haematology except an increase in platelets, slightly decreased plasma transaminases, creatinine, urea and xanthine oxidase activity, without affecting uricemia, glycemia, triglyceridemia and cholesterolemia. GSP did not affect intracellular mediators as calcium, free iron or H2O2, but exerted real anti-oxidative properties in the four selected organs as assessed by lower lipoperoxidation and carbonylation, higher non protein thiols and antioxidant enzyme activities as CAT, GPx and SOD. Besides GSP exerted anti-inflammatory properties as supported by lower plasma IL17 A and CRP and higher IL10 and adiponectin. Histopathologically GSP provoked the dilation of heart and kidney arterioles and increased the size of the hippocampal dentate gyrus reflecting higher neurogenesis as assessed by Ki-67 labeling. Under the experimental conditions of the current study, GSP appeared as highly safe even when administered at very high dosage and could find potential applications in a variety of biotic or abiotic stresses-induced multi-organ dysfunction. |
| Databáze: | OpenAIRE |
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