When Wavelengths Collide: Bias in Cell Abundance Measurements Due to Expressed Fluorescent Proteins
| Autor: | Ariel Hecht, Drew Endy, Matthew S. Munson, Marc L. Salit |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Chemistry Abundance (chemistry) Liquid culture fungi 030106 microbiology Biomedical Engineering General Medicine Optical density Biochemistry Genetics and Molecular Biology (miscellaneous) Fluorescence Luminescent Proteins 03 medical and health sciences Wavelength 030104 developmental biology Cell density Biophysics |
| Zdroj: | ACS Synthetic Biology. 5:1024-1027 |
| ISSN: | 2161-5063 |
| DOI: | 10.1021/acssynbio.6b00072 |
| Popis: | The abundance of bacteria in liquid culture is commonly inferred by measuring optical density at 600 nm. Red fluorescent proteins (RFPs) can strongly absorb light at 600 nm. Increasing RFP expression can falsely inflate apparent cell density and lead to underestimations of mean per-cell fluorescence by up to 10%. Measuring optical density at 700 nm would allow estimation of cell abundance unaffected by the presence of nearly all fluorescent proteins. |
| Databáze: | OpenAIRE |
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