Development of a Sensitive Bioanalytical Method for the Quantification of Lacosamide in Rat Plasma. Application to Preclinical Pharmacokinetics Studies in Rats
| Autor: | SG Vasantharaju, Karthik Arumugam, SK Shah, N Desai, BS Muddukrishna |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Bioanalysis
Lacosamide Liquid-Liquid Extraction High-performance liquid chromatography Pharmacokinetics Liquid–liquid extraction Spectrophotometry Preclinical pharmacokinetics Acetamides Drug Discovery medicine Animals Rats Wistar Chromatography High Pressure Liquid Chromatography medicine.diagnostic_test Chemistry Extraction (chemistry) Reproducibility of Results Hydrogen-Ion Concentration Reference Standards Rats Calibration Solvents Anticonvulsants Indicators and Reagents Spectrophotometry Ultraviolet medicine.drug |
| Zdroj: | Arzneimittelforschung. 62:243-246 |
| ISSN: | 1616-7066 0004-4172 |
| DOI: | 10.1055/s-0032-1301911 |
| Popis: | A sensitive and selective high performance liquid chromatographic (HPLC) method was developed and validated for quantification of lacosamide in rat plasma. A liquid-liquid extraction procedure was optimized to extract lacosamide from rat plasma. Chromatographic separation was accomplished using a reversed phase C18 Hichrom (250×4.6 mm, 5 µm) column with the mobile phase consisting of acetonitrile-phosphate buffer (pH 3.2±0.1; 20 mM) (21:79, v/v) at a flow rate of 1 mL/min. Both intra- and inter day assay precision and accuracy were lower than 15% CV. The lower limit of quantitation was 25 ng/mL for lacosamide and the response was linear in a concentration range from 25 to 10 000 ng/mL. The developed method was successfully used for the preclinical pharmacokinetic study of lacosamide in rats. |
| Databáze: | OpenAIRE |
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