Emerging tools for bioluminescence imaging
| Autor: | Laura Mezzanotte, Chintan Chawda, Giorgia Zambito |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Cell Membrane Permeability Computer science Gene Expression Computational biology 010402 general chemistry 01 natural sciences Biochemistry Cell Line Analytical Chemistry Structure-Activity Relationship 03 medical and health sciences Animals Humans Bioluminescence imaging Bioluminescence Clustered Regularly Interspaced Short Palindromic Repeats Luciferase Gene Knock-In Techniques Luciferases Luminescent Agents Optical Imaging Luciferin 3. Good health 0104 chemical sciences 030104 developmental biology Blood-Brain Barrier Luminescent Measurements Large animal |
| Zdroj: | Current Opinion in Chemical Biology |
| ISSN: | 1367-5931 |
| DOI: | 10.1016/j.cbpa.2021.02.005 |
| Popis: | Bioluminescence (BL) relies on the enzymatic reaction between luciferase, a substrate conventionally named luciferin, and various cofactors. BL imaging has become a widely used technique to interrogate gene expression and cell fate, both in small and large animal models of research. Recent developments include the generation of improved luciferase–luciferin systems for deeper and more sensitive imaging as well as new caged luciferins to report on enzymatic activity and other intracellular functions. Here, we critically evaluate the emerging tools for BL imaging aiming to provide the reader with an updated compendium of the latest developments (2018–2020) and their notable applications. |
| Databáze: | OpenAIRE |
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