Use of particle-loaded membranes to extract steroids for high-performance liquid chromatographic analyses improved analyte stability and detection
| Autor: | Donald A. Wiebe, Carol Onsager, Gary L. Lensmeyer, Ian H. Carlson |
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| Rok vydání: | 1995 |
| Předmět: |
Detection limit
Analyte Chromatography Hydrocortisone Elution Chemistry Prednisolone Organic Chemistry Extraction (chemistry) Membranes Artificial General Medicine Biochemistry High-performance liquid chromatography Sensitivity and Specificity Analytical Chemistry Cortisone Adsorption Membrane Drug Stability Reagent Humans Prednisone Corticosterone Chromatography High Pressure Liquid |
| Zdroj: | Journal of chromatography. A. 691(1-2) |
| ISSN: | 0021-9673 |
| Popis: | Cortisol, cortisone, corticosterone, prednisone and prednisolone are extracted from serum using the novel particle-loaded octyl (C8)-bonded silica in PTFE membrane. Extracts are directly injected, without further concentration, onto a narrow (2.0 mm) or conventional (4.6 mm) bore octyldecyl (C18) HPLC column. Method performance data demonstrate linearity from 0.4 microgram/dl (low limit of detection) up to at least 60 micrograms/dl. Extraction recoveries exceeded 85% and precision (between-run) R.S.D.s averaged < 5%. Interferences were minimal and selectivity was improved over conventional immunochemical steroid assays. When compared to large particle sorbents packed in columns or to traditional liquid-liquid extractions, the membrane extracted steroids in less time, used less reagent, and had smaller elution volumes, thereby obviating steroid instability/adsorption problems associated with traditional concentrating techniques required to improve analytical sensitivity. |
| Databáze: | OpenAIRE |
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