Cubosomes for in vivo fluorescence lifetime imaging
| Autor: | Sergio Murgia, Stefania Biffi, Claudia Caltagirone, Yeshayahu Talmon, Antonella Rosa, Marc Obiols-Rabasa, Angela Maria Falchi, Judith Schmidt, Chiara Garrovo, Maura Monduzzi, Laura Andolfi, Paolo Macor, Luca Petrizza, Andrea Lorenzon, Vito Lippolis, Valeria Meli, Luca Prodi |
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| Přispěvatelé: | Biffi, Stefania, Andolfi, Laura, Caltagirone, Claudia, Garrovo, Chiara, Falchi, Angela M., Lippolis, Vito, Lorenzon, Andrea, Macor, Paolo, Meli, Valeria, Monduzzi, Maura, Obiols Rabasa, Marc, Petrizza, Luca, Prodi, Luca, Rosa, Antonella, Schmidt, Judith, Talmon, Yeshayahu, Murgia, Sergio, Obiols-Rabasa, Marc |
| Rok vydání: | 2016 |
| Předmět: |
Erythrocytes
02 engineering and technology Photochemistry 01 natural sciences Mice liquid crystalline nanoparticle General Materials Science Mice Inbred BALB C Spectroscopy Near-Infrared Chemistry (all) Optical Imaging Carbocyanines 021001 nanoscience & nanotechnology Fluorescence Liver liquid crystalline nanoparticles Mechanics of Materials Injections Intravenous Female fluorescence optical imaging in vivo Materials Science (all) 0210 nano-technology Materials science Nanostructure Cell Survival Drug Compounding Bioengineering 010402 general chemistry fluorescence lifetime probe Time-Lapse Imaging Glycerides Optical imaging Animal model In vivo In vivo fluorescence Animals Humans Mechanics of Material Electrical and Electronic Engineering Particle Size Nir fluorescence Fluorescent Dyes Mechanical Engineering General Chemistry 0104 chemical sciences Liposomes Biophysics NIH 3T3 Cells Nanoparticles |
| Zdroj: | Nanotechnology (Bristol, Online) 28 (2017). doi:10.1088/1361-6528/28/5/055102 info:cnr-pdr/source/autori:Biffi S.; Andolfi L.; Caltagirone C.; Garrovo C.; Falchi A.M.; Lippolis V.; Lorenzon A.; Macor P.; Meli V.; Monduzzi M.; Obiols-Rabasa M.; Petrizza L.; Prodi L.; Rosa A.; Schmidt J.; Talmon Y.; Murgia S./titolo:Cubosomes for in vivo fluorescence lifetime imaging/doi:10.1088%2F1361-6528%2F28%2F5%2F055102/rivista:Nanotechnology (Bristol, Online)/anno:2017/pagina_da:/pagina_a:/intervallo_pagine:/volume:28 |
| ISSN: | 1361-6528 |
| Popis: | Herein we provided the first proof of principle for in vivo fluorescence optical imaging application using monoolein-based cubosomes in a healthy mouse animal model. This formulation, administered at a non-cytotoxic concentration, was capable of providing both exogenous contrast for NIR fluorescence imaging with very high efficiency and chemospecific information upon lifetime analysis. Time-resolved measurements of fluorescence after the intravenous injection of cubosomes revealed that the dye rapidly accumulated mainly in the liver, while lifetimes profiles obtained in vivo allowed for discriminating between free dye or dye embedded within the cubosome nanostructure after injection. |
| Databáze: | OpenAIRE |
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