Tenascin-C Modulates Adhesion of Cardiomyocytes to Extracellular Matrix during Tissue Remodeling after Myocardial Infarction
| Autor: | Kyoko Imanaka-Yoshida, Takashi Shimojo, Michiaki Hiroe, Yoshiko Ohta, Shigeru Ishiyama, Teruyo Sakakura, Toshimichi Yoshida, Toshio Nishikawa |
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| Rok vydání: | 2001 |
| Předmět: |
Male
Pathology medicine.medical_specialty Blotting Western Myocardial Infarction Fluorescent Antibody Technique Connective tissue Tenascin In situ hybridization Pathology and Forensic Medicine Extracellular matrix Laminin Cell Adhesion Tumor Cells Cultured medicine Animals Humans Myocyte RNA Messenger Rats Wistar Ventricular remodeling Molecular Biology biology business.industry Myocardium Tenascin C Cell Biology musculoskeletal system medicine.disease Immunohistochemistry Extracellular Matrix Rats medicine.anatomical_structure biology.protein business |
| Zdroj: | ResearcherID |
| ISSN: | 0023-6837 |
| DOI: | 10.1038/labinvest.3780313 |
| Popis: | Tenascin-C (TNC), an extracellular matrix glycoprotein, plays important roles in tissue remodeling. TNC is not normally expressed in adults but reappears under pathologic conditions. The present study was designed to clarify the contribution of TNC to ventricular remodeling after myocardial infarction. We examined the expression of TNC after experimental myocardial infarction in the rat by immunohistochemistry and in situ hybridization. Within 24 hours of permanent coronary ligation, interstitial fibroblasts in the border zone started to express TNC mRNA. The expression of TNC was down-regulated on Day 7 and was no longer apparent by Day 14 after infarction. During the healing process, TNC protein and TNC-producing cells were found at the edges of the residual myocardium. Some of the TNC-producing cells were immunoreactive for alpha-smooth muscle actin. In culture, TNC increased the number of cardiomyocytes attached to laminin but inhibited the formation of focal contacts at costameres. The results indicate that during the acute phase after myocardial infarction, interstitial cells in the border zone synthesize TNC, which may loosen the strong adhesion of surviving cardiomyocytes to connective tissue and thereby facilitate tissue reorganization. |
| Databáze: | OpenAIRE |
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