| Autor: |
X. Shirley Liu, Bo Li, Ming Tang, Heng Li, Kenneth J. Livak, Xihao Hu, Gali Bai, Jennifer Altreuter, Yang Cao, David Cohen, Zhangyi Ouyang, Li Song |
| Rok vydání: |
2023 |
| ISSN: |
2326-6066 |
| DOI: |
10.1158/2326-6066.c.6550716.v1 |
| Popis: |
We applied our computational algorithm TRUST4 to assemble immune receptor (T-cell receptor/B-cell receptor) repertoires from approximately 12,000 RNA sequencing samples from The Cancer Genome Atlas and seven immunotherapy studies. From over 35 million assembled complete complementary-determining region 3 sequences, we observed that the expression of CCL5 and MZB1 is the most positively correlated genes with T-cell clonal expansion and B-cell clonal expansion, respectively. We analyzed amino acid evolution during B-cell receptor somatic hypermutation and identified tyrosine as the preferred residue. We found that IgG1+IgG3 antibodies together with FcRn were associated with complement-dependent cytotoxicity and antibody-dependent cellular cytotoxicity or phagocytosis. In addition to B-cell infiltration, we discovered that B-cell clonal expansion and IgG1+IgG3 antibodies are also correlated with better patient outcomes. Finally, we created a website, VisualizIRR, for users to interactively explore and visualize the immune repertoires in this study.See related Spotlight by Liu and Han, p. 786 |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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