A useful cell system for studying the regulation of 17HSD/KSR type 2 activity and expression in ovarian epithelial cancer
| Autor: | Charles H. Blomquist, Pierre R. Provost, Paul H. Lima, Yves Tremblay |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
medicine.medical_specialty
17-Hydroxysteroid Dehydrogenases Endocrinology Diabetes and Metabolism Blotting Western Clinical Biochemistry Adenocarcinoma Biology Reductase Biochemistry Endocrinology Cell Line Tumor Internal medicine medicine Humans RNA Messenger Molecular Biology Ovarian Neoplasms Regulation of gene expression Messenger RNA Cancer Epithelial Cells Cell Biology medicine.disease Molecular biology Gene Expression Regulation Neoplastic Blot Cell culture Molecular Medicine Female Ovarian cancer Fetal bovine serum |
| Zdroj: | The Journal of Steroid Biochemistry and Molecular Biology. 122:295-301 |
| ISSN: | 0960-0760 |
| Popis: | 17β-Hydroxysteroid dehydrogenase/17-ketosteroid reductase (17HSD/KSR) activity and 17HSD/KSR types 1, 2, 4, and 5 mRNA levels were characterized in ovarian cancer cell lines derived from patients unexposed to radiation or chemotherapy. Activity was at the limit of detection in TOV-112D and TOV-21G cells. Activity in OV-90 was comparable to that in human placental tissue, was predominantly microsomal and was 17HSD/KSR type 2-like in substrate specificity and inhibition patterns. In monolayers, conversion of testosterone (T) to androstenedione (A) was 12-fold greater than that of A to T. Reduction of fetal bovine serum to 0.3% in the culture medium had no effect on 17β-HSD activity. Significant levels of type 1 and type 2 mRNAs were observed in OV-90 while only trace amounts were detected in TOV-21G. In contrast, type 4 mRNA levels were comparable for OV-90 and TOV-21G. Type 5 mRNA was detected in both cell lines but its level in OV-90 was twice that of TOV-21G. In OV-90, the type 2-like activity was predominant even though the type 5 mRNA level was 2.5-fold higher than that of the type 2. OV-90 cells may be a useful system for studying the regulation of 17HSD/KSR type 2 activity and expression in ovarian epithelial cancer. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect