Characterization of multi-drug resistantMycobacterium tuberculosisfrom immigrants residing in the USA using Ion Torrent full-gene sequencing
| Autor: | Luke T. Daum, M. Khubbar, P. Hunter, M. S. Gradus, J. Sromek, Sanjib Bhattacharyya, Gerald W. Fischer |
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| Rok vydání: | 2013 |
| Předmět: |
China
Tuberculosis Epidemiology Molecular Sequence Data Antitubercular Agents Short Report Emigrants and Immigrants India Myanmar Drug resistance Ion Torrent sequencing Genetic analysis Genome DNA sequencing Mycobacterium tuberculosis Wisconsin Bacterial Proteins Drug Resistance Multiple Bacterial Tuberculosis Multidrug-Resistant MDR medicine Humans Drug resistance genes Gene Genetics Bacteriological Techniques biology High-Throughput Nucleotide Sequencing Ion semiconductor sequencing medicine.disease biology.organism_classification Infectious Diseases next-generation sequencing Genome Bacterial |
| Zdroj: | Epidemiology and Infection |
| ISSN: | 1469-4409 0950-2688 |
| DOI: | 10.1017/s0950268813002409 |
| Popis: | SUMMARYDrug-resistantMycobacterium tuberculosisbacterium (MTB) is spreading worldwide. Three drug-resistant isolates were detected in Burmese, Hmong, and Indian immigrants currently residing in Milwaukee, Wisconsin, USA. Ion Torrent full-gene sequencing and complete genetic analysis was performed within 5 days and compared to results from traditional drug sensitivity testing (DST). Genetic characterization of seven, full-length resistance-associated genes revealed two MDR and one highly resistant strain with important drug-resistant mutations that were confirmed by traditional DST. The rapid turnaround from sample-to-sequence underscores the public health value of Ion Torrent full-gene sequencing of MDR/XDR genes from epidemiologically significant clinical isolates. |
| Databáze: | OpenAIRE |
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