Detection of acute hepatitis C virus infection by ELISA using a synthetic peptide comprising a structural epitope
| Autor: | B M Baroudy, G J Kotwal, Jerome B. Zeldis, F F McDonald, I K Kuramoto, Paul V. Holland, G M Schiff |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
Hepatitis C virus
Immunoblotting Molecular Sequence Data Enzyme-Linked Immunosorbent Assay Biology medicine.disease_cause Epitope Virus Epitopes Antigen Reference Values medicine Humans Amino Acid Sequence Hepatitis Antibodies Viral Structural Proteins Hepatitis Hepatitis B virus Multidisciplinary virus diseases Hepatitis C Hepatitis C Antibodies medicine.disease Virology Molecular biology digestive system diseases Acute Disease biology.protein Antibody Peptides Research Article |
| Zdroj: | Proceedings of the National Academy of Sciences. 89:4486-4489 |
| ISSN: | 1091-6490 0027-8424 |
| DOI: | 10.1073/pnas.89.10.4486 |
| Popis: | An enzyme-linked immunosorbent assay (ELISA) was developed by using a synthetic polypeptide (SP) whose sequence was derived from the structural region of hepatitis C virus (HCV). Results of several coded panels of sera obtained from volunteer blood donors and patients with apparent non-A, non-B hepatitis and/or hepatitis B virus used in this ELISA were compared with those of a commercially available first-generation C-100 ELISA (using nonstructural HCV antigens), an experimental second-generation C-200/C-22 ELISA (using both structural and nonstructural HCV antigens), and recombinant immunoblot assays RIBA-I and RIBA-II. In the majority of cases, the results obtained with the HCV-SP ELISA correlated well with those obtained by RIBA-II and C-200/C-22 ELISA. In contrast, many samples that were repeatedly reactive in the C-100 ELISA results were nonreactive with RIBA and HCV-SP ELISA. In addition, HCV-SP detected HCV-specific antibody that appeared within a month of infection and coincided with the earliest increase in alanine aminotransferase. In summary, we have developed an ELISA based on a structural HCV synthetic polypeptide, HCV-SP, that has high specificity and sensitivity and is capable of detecting specific antibodies in the acute phase of HCV infection. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|