The application of electrospray and neutral-loss mass spectrometry to the identification of the metabolites of S12813 in rat liver slices
| Autor: | Albert Taylor, Robin Brownsill, Jean‐Philippe Combal, Marc Bertrand, Bernard Walther, D. E. Games, Wim Luijten |
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| Rok vydání: | 1994 |
| Předmět: |
Male
Electrospray Electrospray ionization Glucuronates In Vitro Techniques Mass spectrometry High-performance liquid chromatography Mass Spectrometry Analytical Chemistry chemistry.chemical_compound Pyridine Animals Rats Wistar Oxazoles Biotransformation Spectroscopy Analgesics Chromatography Sulfates Chemistry Organic Chemistry Metabolism Rats Metabolic pathway Liver Glucuronide Chromatography Liquid |
| Zdroj: | Rapid Communications in Mass Spectrometry. 8:361-365 |
| ISSN: | 1097-0231 0951-4198 |
| DOI: | 10.1002/rcm.1290080504 |
| Popis: | The metabolism of S12813, (3-{2-[4-phenyl piperazin-1-yl] ethyl}-2-oxo-2,3-dihydro oxazolo [4, 5-b] pyridine chlorohydrate), in rat liver slice incubates was examined by high performance liquid chromatography combined with mass spectrometry. Electrospray ionization was used together with tandem mass spectrometric techniques of analysis (MS/MS). Polar phase I and phase II metabolites were identified as C-oxidation products, which were then conjugated to form either sulphate or glucuronide metabolites. On the basis of the identifications made, a metabolic pathway of S12813 in rat liver slices has been proposed. |
| Databáze: | OpenAIRE |
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