Crystallization and preliminary X-ray study of the D-altritol oligonucleotide GTGTACAC
| Autor: | Luc Van Meervelt, Margriet Ovaere, Arthur Van Aerschot, Piet Herdewijn, Mikhail Abramov |
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| Rok vydání: | 2010 |
| Předmět: |
Base Sequence
Chemistry Oligonucleotide Resolution (electron density) Biophysics Oligonucleotides Crystal structure Condensed Matter Physics Ring (chemistry) Crystallography X-Ray Biochemistry law.invention chemistry.chemical_compound Crystallography Sugar Alcohols Structural Biology law Crystallization Communications Ribose Genetics Nucleic acid Histone octamer Crystallization |
| Zdroj: | Acta crystallographica. Section F, Structural biology and crystallization communications. 66(Pt 4) |
| ISSN: | 1744-3091 |
| Popis: | In altritol nucleic acids (ANAs), the natural five-membered ribose ring of RNA is replaced by the six-membered D-altritol ring. ANAs are good candidates to act as siRNAs in the RNA-interference pathway. Crystals of the fully modified altritol self-complementary octamer GTGTACAC were grown by the hanging-drop vapour-diffusion technique at 289 K. Diffraction data were recorded on SLS beamline X06DA and processed to 3.0 A resolution. The crystals belonged to the hexagonal space group P6(1)22 or P6(5)22, with unit-cell parameters a = 25.05, c = 117.58 A. |
| Databáze: | OpenAIRE |
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