Supplemental Figures 1 to 4 from MYC Targeted Long Noncoding RNA DANCR Promotes Cancer in Part by Reducing p21 Levels

Autor: Chi V. Dang, Lin Zhang, Anil K. Sood, Angelo M. DeMarzo, Gabriel Lopez-Berestein, Cristian Rodriguez-Aguayo, Sunila Pradeep, Youyou Zhang, Yan Xiang, Dahai Jiang, Xiaowen Hu, Zachary E. Stine, Lingegowda S. Mangala, Zhongyi Hu, Yunqi Lu
Rok vydání: 2023
Popis: Figure S1. The expression of DANCR is upregulated by MYC activation in P493-6 cells Normalized expression of DANCR (KIAA0114) in MYC-OFF or MYC-ON P493-6 cells measured by exon microarrays (PLoS One 6, e26057). Three independent array experiments were performed. Figure S2. Human-mouse sequence alignment analysis of DANCR and mDancr Figure S3. The mRNA expression of the DANCR adjacent protein-coding genes in cells transduced with DANCR-specific siRNA Control and DANCR siRNAs were transduced into PC3 cells. After 48 and 72 hours, the mRNA expression levels of the DANCR adjacent protein-coding genes (ERVMER34-1 and UPS46) were analyzed by qRT-PCR. Figure S4. Effects of two DANCR targeting antisense morpholinos on P493-6 cell proliferation and p21 expression. A. RT-PCR analysis of DANCR expression in P493-6 after being treated by various concentrations of the two DANCR antisense morpholinos. B. Effects of two different morpholinos targeting DANCR on proliferation of P493-6 cells as compared with control morpholino. C. Induction of P21 by loss of DANCR expression in P493-6 cells treated by two concentrations of the two DANCR antisense morpholinos.
Databáze: OpenAIRE