Human Synovia Contains Trefoil Factor Family (TFF) Peptides 1–3 Although Synovial Membrane Only Produces TFF3: Implications in Osteoarthritis and Rheumatoid Arthritis

Autor: Horst Claassen, Dankwart Stiller, Michael Tsokos, Judith Popp, Saskia Etzold, Martin Schicht, Friedrich Paulsen, Fabian Garreis, Kolja Gelse, Patricia Klinger, Stefan Sesselmann
Jazyk: angličtina
Rok vydání: 2019
Předmět:
0301 basic medicine
Male
Osteoarthritis
Arthritis
Rheumatoid
0302 clinical medicine
Blood serum
osteoarthritis (OA)
Synovial Fluid
rheumatoid arthritis (RA)
synovial membrane (SM)
Spectroscopy
Aged
80 and over
synovial fluid (SF)
medicine.diagnostic_test
Synovial Membrane
General Medicine
Middle Aged
Tissue Donors
Computer Science Applications
medicine.anatomical_structure
030220 oncology & carcinogenesis
Rheumatoid arthritis
Immunohistochemistry
Female
Trefoil Factor-1
Trefoil Factor-2
Trefoil Factor-3
Adult
medicine.medical_specialty
Catalysis
Article
Inorganic Chemistry
03 medical and health sciences
Western blot
Internal medicine
Joint capsule
medicine
Synovial fluid
Humans
ddc:610
RNA
Messenger
Physical and Theoretical Chemistry
Molecular Biology
Aged
business.industry
Organic Chemistry
trefoil factor family peptides (TFF)
medicine.disease
030104 developmental biology
Endocrinology
Synovial membrane
business
Peptides
Zdroj: International Journal of Molecular Sciences
Volume 20
Issue 23
ISSN: 1422-0067
Popis: Objective: Trefoil factor family peptide 3 (TFF3) has been shown to support catabolic functions in cases of osteoarthritis (OA). As in joint physiology and diseases such as OA, the synovial membrane (SM) of the joint capsule also plays a central role. We analyze the ability of SM to produce TFF compare healthy SM and its secretion product synovial fluid (SF) with SM and SF from patients suffering from OA or rheumatoid arthritis (RA). Methods: Real-time PCR and ELISA were used to measure the expression of TFFs in healthy SM and SM from patients suffering from OA or RA. For tissue localization, we investigated TFF1-3 in differently aged human SM of healthy donors by means of immunohistochemistry, real-time PCR and Western blot. Results: Only TFF3 but not TFF1 and -2 was expressed in SM from healthy donors as well as cases of OA or RA on protein and mRNA level. In contrast, all three TFFs were detected in all samples of SF on the protein level. No significant changes were observed for TFF1 at all. TFF2 was significantly upregulated in RA samples in comparison to OA samples. TFF3 protein was significantly downregulated in OA samples in comparison to healthy samples and cases of RA significantly upregulated compared to OA. In contrast, in SM TFF3 protein was not significantly regulated. Conclusion: The data demonstrate the production of TFF3 in SM. Unexpectedly, SF contains all three known TFF peptides. As neither articular cartilage nor SM produce TFF1 and TFF2, we speculate that these originate with high probability from blood serum.
Databáze: OpenAIRE
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