Detection of Different Quasispecies of Hepatitis C Virus Core Region in Cancerous and Noncancerous Lesions
| Autor: | Katsuhiko Yoshimoto, Takahiro Horie, Shiro Yogita, Ichiro Shimizu, Seiki Tashiro, Chiyo Horie, Susumu Ito, Hiroyuki Iwahana, Mitsuo Itakura |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Liver Cirrhosis
Male Carcinoma Hepatocellular Hepatitis C virus Molecular Sequence Data Biophysics Peptide Viral quasispecies Biology medicine.disease_cause Biochemistry DNA sequencing medicine Humans Hepatitis C virus core Nucleotide Amino Acid Sequence Molecular Biology Polymorphism Single-Stranded Conformational Aged DNA Primers chemistry.chemical_classification Base Sequence Sequence Homology Amino Acid Viral Core Proteins Liver Neoplasms Cell Biology Middle Aged medicine.disease Hepatitis C Virology Molecular biology digestive system diseases Amino acid chemistry Hepatocellular carcinoma DNA Viral Sequence Alignment |
| Zdroj: | Biochemical and Biophysical Research Communications. 218:674-681 |
| ISSN: | 0006-291X |
| DOI: | 10.1006/bbrc.1996.0121 |
| Popis: | The quasispecies of hepatitis C virus (HCV) core region in non-cancerous and cancerous hepatocellular carcinoma (HCC) lesions, respectively, of 7 patients with hepatocellular carcinoma were studied. Multiple fluorescence based-polymerase chain reaction (PCR)-single strand conformation polymorphism exhibited a different set and a larger number of quasispecies in cancerous portions than those in non-cancerous portions. DNA sequencing of PCR-amplified core region substantiated an accumulation of nucleotide substitutions, and a greater number of quasispecies in cancerous portions than those in non-cancerous portions. The deduced amino acid sequences disclosed that at the peptide position 45, Ser is dominant in non-cancerous lesions, and Gly in cancerous lesions, respectively. Thus, HCV in hepatocellular carcinoma includes a large number of specific quasispecies presumably due to their vigorous proliferation. A different set of quasispecies with the amino acid change is presumed to be related to the hepatocarcinogenesis. |
| Databáze: | OpenAIRE |
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