Effect of hepatocyte growth factor (HGF) on the level of SurvivinXIAP expression in several human cancer cell lines, after treating with DNA damaging agent
| Autor: | Ramin Sarrami-Forooshani, Mansour Salehi, A Oghalaei, Kianoosh Keyhanian, Nayere Askary, Rosita Edalat, Mohammad Ali Shokrgozar, Arghavan Golshani |
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| Rok vydání: | 2007 |
| Předmět: |
Programmed cell death
Survivin Clinical Biochemistry X-Linked Inhibitor of Apoptosis Protein Biology Inhibitor of Apoptosis Proteins Cell Line Tumor Neoplasms medicine Humans MTT assay Molecular Biology Cell Death Cell growth Cytotoxins Hepatocyte Growth Factor Cell Biology General Medicine Molecular biology XIAP Neoplasm Proteins Gene Expression Regulation Neoplastic Apoptosis Cell culture Cytoprotection Doxorubicin Drug Resistance Neoplasm Cancer research Hepatocyte growth factor Microtubule-Associated Proteins medicine.drug DNA Damage |
| Zdroj: | Molecular and cellular biochemistry. 304(1-2) |
| ISSN: | 0300-8177 |
| Popis: | Hepatocyte growth factor (HGF) has opposite biological activities in regulating apoptosis, also underlying molecular mechanisms are not clearly defined. We investigated HGF ability to inhibit cell death, which was induced by Doxorubicin, a DNA damaging agent. Also Survivin and XIAP mRNA levels were compared in HGF treated and non-treated cells. Cell proliferation and death were assessed using MTT assay and dye exclusion tests. Quantitative real-time PCR was used to evaluate Survivin and XIAP expression levels after treatment with HGF. ELISA was performed to quantify HGF secretion in the selected cancer cell lines media. HGF appeared to have inhibitory effect on Doxorubicin induced cell death in all of the studied cell lines. It had minimal effect on XAIP and Survivin expression levels in MRC-5, MOLT-4 and AGS cell lines; except for XIAP expression level in AGS cell line, which was increased substantially after treatment. Surprisingly, in KG-1 cell line, XIAP and Survivin expression levels were significantly reduced after HGF treatment. Although several members of IAP gene family are reported to play role in HGF mediated cytoprotective pathway, we showed that XIAP and Survivin do not seem to be involved. |
| Databáze: | OpenAIRE |
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