DnaK from Bifidobacterium animalis subsp. lactis is a surface-exposed human plasminogen receptor upregulated in response to bile salts
| Autor: | Jessica Fiori, Manuela Centanni, Silvia Turroni, Elena Biagi, Marco Candela, Sven Hammerschmidt, Patrizia Brigidi, Simone Bergmann, Catia Orrico |
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| Přispěvatelé: | Department of Pharmaceutical Sciences, University of Bologna, Italy., Candela M., Centanni M., Fiori J., Biagi E., Turroni S., Orrico C., Bergmann S., Hammerschmidt S., Brigidi P. |
| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: |
Proteome
Bifidobacteriales Microbiology Actinobacteridae Bacterial cell structure law.invention Bile Acids and Salts Probiotic Bacterial Proteins Microscopy Electron Transmission law Humans HSP70 Heat-Shock Proteins Receptor biology Plasminogen biology.organism_classification Recombinant Proteins Bifidobacterium animalis Up-Regulation Bifidobacteriaceae Gastrointestinal Tract Biochemistry Phosphopyruvate Hydratase bacteria Bifidobacterium Bacteria |
| Popis: | Bifidobacteriumanimalissubsp.lactislives in the gastrointestinal tract of most mammals, including humans. Recently, for the probiotic strainB. animalissubsp.lactisBI07, a dose-dependent plasminogen-binding activity was demonstrated and five putative plasminogen-binding proteins were identified. Here we investigated the role of surface DnaK as aB. animalissubsp.lactisBI07 plasminogen receptor. DnaK was visualized on the bacterial cell surface by transmission electron microscopy. The His-tagged recombinant DnaK protein showed a high affinity for human plasminogen, with an equilibrium dissociation constant in the nanomolar range. The capability to tolerate physiological concentrations of bile salts is a crucial feature for an intestinal symbiont micro-organism. By proteome analysis we demonstrated that the long-term exposure ofB. animalissubsp.lactisBI07 to bile salts results in the upregulation of important surface plasminogen receptors such as DnaK and enolase. Moreover, adaptation ofB. animalissubsp.lactisBI07 to physiological concentrations of bile salts significantly increased its capacity to interact with the host plasminogen system. By enhancing the bacterial capacity to interact with the host plasminogen, the gut bile environment may facilitate the colonization of the human host byB. animalissubsp.lactisBI07. |
| Databáze: | OpenAIRE |
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