A rapid and simple protocol for the isolation of bacteriophages from coastal organisms
| Autor: | Alex Echeverría-Vega, Felipe Gordillo-Fuenzalida, Camila Saez-Saavedra, Rubén Araya, Pablo Morales-Vicencio |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Phage prospection
Clinical Biochemistry 010501 environmental sciences medicine.disease_cause 01 natural sciences Microbiology 03 medical and health sciences Water column Aquaculture medicine Vibrio ordalii lcsh:Science ComputingMethodologies_COMPUTERGRAPHICS 030304 developmental biology 0105 earth and related environmental sciences Immunology and Microbiology 0303 health sciences biology business.industry Vibrio bacteriophage fungi Pathogenic bacteria Phage isolation biology.organism_classification Isolation (microbiology) Antimicrobial Medical Laboratory Technology Lytic cycle lcsh:Q business Bacteria Isolation of bacteriophages from coastal zone |
| Zdroj: | MethodsX MethodsX, Vol 6, Iss, Pp 2614-2619 (2019) |
| ISSN: | 2215-0161 |
| DOI: | 10.1016/j.mex.2019.11.003 |
| Popis: | Graphical abstract This work details a protocol for recovering bacteriophages from intertidal sessile mussels and testing their lytic activity against pathogenic bacteria. Although bacteriophages were highly abundant in coastal filter-feeding organisms, they were not detectable in the surrounding water column. This difference reflects the high filtering rate of the mussels, which capture and concentrate high amounts of bacteria, generating an ideal environment for bacteriophages. We validated the protocol providing a mean concentration of 4E + 04 PFU mL-1 lytic bacteriophages for the fish-pathogen bacterium Vibrio ordalii. We suggest that this method has particular utility for the recovery of bacteriophages for use as natural antimicrobial agents in aquaculture. |
| Databáze: | OpenAIRE |
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