Differential expression of putative adhesin genes of Actinobacillus suis grown in in vivo -like conditions
| Autor: | Mario Jacques, Adina R. Bujold, Josée Labrie, Janet I. MacInnes |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Bacteriological Techniques General Veterinary biology 030106 microbiology Mutant Biofilm Wild type Actinobacillus suis Gene Expression Regulation Bacterial General Medicine biochemical phenomena metabolism and nutrition biology.organism_classification Microbiology Bacterial adhesin 03 medical and health sciences 030104 developmental biology Biofilms Pilin biology.protein Adhesins Bacterial Bacterial outer membrane Gene |
| Zdroj: | Veterinary Microbiology. 195:60-69 |
| ISSN: | 0378-1135 |
| DOI: | 10.1016/j.vetmic.2016.09.005 |
| Popis: | Actinobacillus suis is an opportunistic pathogen that resides in the tonsils of the soft palate of swine. Unknown stimuli can cause this organism to invade the host, resulting in septicaemia and sequelae including death. To better understand its pathogenesis, the expression of several adhesin genes was evaluated by semi-quantitative real-time PCR in A. suis grown in conditions that mimic the host environment, including different nutrient and oxygen levels, exponential and stationary phases of growth, and in the presence of the stress hormone epinephrine. Fifty micromolar epinephrine did not affect the growth rate or expression of A. suis adhesin genes, but there was a significant growth phase effect for many genes. Most adhesin genes were also differentially expressed during anoxic static growth or aerobic growth, and in this study, all genes were differentially expressed in either exponential or stationary phase. Based on the time*treatment interactions observed in the anoxic study, a model of persistence of A. suis in the host environment in biofilm and planktonic states is proposed. Biofilm dynamics were further studied using wild type and isogenic mutants of the type IVb pilin (Δ flp1) , the OmpA outer membrane protein ( ΔompA) , and the fibronectin-binding ( ΔcomE1) genes. Disruption of these adhesin genes affected the early stages of biofilm formation, but in most cases, biofilm formation of the mutant strains was similar to that of the wild type by 24 h of incubation. We postulate that other adhesins may have overlapping functions that can compensate for those of the missing adhesins. |
| Databáze: | OpenAIRE |
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