Differentiation of hamster liver oval cell following Clonorchis sinensis infection
| Autor: | Kyung-Hwan Joo, Dae Yong Kim, Yong-Soon Lee, Kwon Hur, Su-Youn Jung, Byung-Il Yoon, Jae-Hyun Lee |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Male
Pathology medicine.medical_specialty Alkylating Agents Liver cytology Liver Diseases Parasitic Hamster Rodent Diseases Albumins Cricetinae medicine Animals Diethylnitrosamine Progenitor cell Clonorchis sinensis General Veterinary biology Mesocricetus Bile duct Endoplasmic reticulum 2-Acetylaminofluorene biology.organism_classification Molecular biology Immunohistochemistry Microscopy Electron medicine.anatomical_structure Liver Hepatocyte Carcinogens Clonorchiasis Keratins alpha-Fetoproteins |
| Zdroj: | The Journal of veterinary medical science. 62(12) |
| ISSN: | 0916-7250 |
| Popis: | Oval cells which appear in the liver after hepatic injuries are suspected to be progenitor cells for both hepatocytes and bile duct cells. Oval cell isolated from the livers of the hamsters treated with diethylnitrosamine and 2-acetylaminofluorene and infected with Clonorchis sinensis (CS). cultured for 2 weeks and evaluated for differentiation and plasticity by electron microscopy and immunohistochemistry. In the CS-uninfected group, glycogen granules and peroxisomes were noted in the cells that were cultured for 2 weeks. Starting at 1 week postculture, immunoreactivity of the cells to cytokeratin 19 markedly decreased but that to albumin and alpha-fetoprotein gradually increased. This means that oval cells isolated from hamsters that were not infected with CS differentiated toward hepatocyte lineage. However, in the CS-infected group, cultured cells contained numerous rough endoplasmic reticulum and showed immunoreactivity that was generally in reverse to that of CS-uninfected group, meaning that cells isolated following CS infection were primed by CS and differentiated toward bile duct cell lineage. The results of this study suggested that oval cells are indeed bipolar progenitor cells for hepatocytes and bile duct cells and can differentiate toward either lineage depending upon the priming factor. |
| Databáze: | OpenAIRE |
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